Technical Library

Recent Entries in Technical Library

• Can I spray the Spore-EX Spray on plexiglass surfaces without damaging it?

  This spray is compatible with plastic/plexiglass. However it will leave residue as it is not an alcohol based formulation, so you may need to wipe dry with a paper towel.

  Related Links and Documents

  • Spore-EX Spray
• I received 3 DetachKits yesterday. Some of the tubes have different colors, although they all come from the same batch.

  The components of the PromoCell DetachKit may occasionally arrive with a non-uniform color appearance. This phenomenon is known to PromoCell’s Quality Assurance Dept.
  It is reversible and has no influence on the quality of the product.

  Related Links and Documents

  • PromoCello DetachKit
• Are endothelial cells α-SMA-negative under all circumstances?

  Scientific findings from different groups, as well as our own results indicate that the presence or absence of α-SMA is not a valid indicator for the composition of a given cell population. Research findings and our inhouse data suggest that endothelial cells are not α-SMA-negative under all circumstances. α-SMA negativity is not an intrinsic property of endothelial cells but can vary depending on extrinsic influences.

  Lot-specific values for α-SMA in our Certificates of Analysis (CoAs) are therefore not considered meaningful.

  Related Links and Documents

  • Endothelial Cell Culture
  • Ando et al.; Am J Physio l. 1999 May;276(5):H1755-68
  • Lu et al.; Stem Cells Dev . 2004 Oct;13(5):521-7
  • Azuma et al.; Biochem Biophys Res Commun . 2009 Mar 13;380(3):620-6
  • Frid et al.; Circ Res . 2002 Jun 14;90(11):1189-96
  • Kovacic et al.; J Am Coll Cardiol . 2019 Jan 22;73(2):190-209
  • Jones et al.; Am J Respir Cell Mol Biol . 1999 Apr;20(4):582-94
  • Ma et al.; Front Cell Dev Biol . 2020 Apr 21;8:260
  • Kocher and Madri; In Vitro Cell Dev Biol . 1989 May;25(5):424-34
  • Amberger et al.; FEBS Lett . 1991 Aug 5;287(1-2):223-5
• Is vWF a great marker for Endothelial Cells?

  Secretory granules called Weibel-Palade bodies (WPBs) containing Von Willebrand factor (vWF) are more linked to the formation of a confluent endothelial monolayer. Research shows that vWF expression is dynamic and highly dependent on the cell culture conditions such as confluence and passage number.
  Therefore, having vWF as a quality control marker for each lot of ECs is not really necessary.

  Related Links and Documents

  • Endothelial Cell Culture
  • Howell et al.; Mol Membr Biol. Nov-Dec 2004;21(6):413-21
• The components of my DetachKit show different colors. Is this normal or does it affect their quality?

  The components of the PromoCell DetachKit may arrive on occasion with a non-uniform color appearance. This phenomenon is known by PromoCell’s Quality Assurance. It is reversible and does not influence the quality of the product.

  Related Links and Documents

  • PromoCell DetachKit
  • PromoCell DetachKit 2
• The supplements arrived defrosted. Are they still ok?

  PromoCell supplements (SupplementMixes and SupplementPacks) are routinely shipped with dry ice. On occasion, the supplements may arrive defrosted. You can either use them immediately to prepare fresh growth media or refreeze them at -20°C. We have performed extensive stability tests and can guarantee that the supplements are stable for up to 1 week at room temperature.

  Sole exception: The SupplementMix (C-39420) of our Melanocyte Growth Medium M2 is less stable and should no longer be used when it arrived thawed, at room temperature.

  Related Links and Documents

  • Media for Human Primary Cell Culture
  • Melanocyte Growth Medium M2
• Can you please let me know whether Spore-EX Spray can eliminate DNA and RNA from surfaces?

  Yes, our Spore-EX Spray (PK-CC91-5053) is also suitable for denaturing DNA and RNA.

  Related Links and Documents

  • Spore-EX Spray
• What is the Endotoxin level of PromoCell’s PBS?

  The specification of endotoxin level is < 1 EU/ml.
  For all our produced batches, the result is 0.005 EU/ml.

  Related Links and Documents

  • Dulbecco’s PBS
• Can I defrost Accutase Solution, prepare aliquots and refreeze them?

  Yes, Accutase Solution can be defrosted, aliquoted, and then refrozen.

  Defrosting: Accutase should be defrosted overnight in the refrigerator or placed in a tub of cold tap water.  Do not defrost in a 37°C water bath.
  Stability: Once thawed, it is stable for at least 2 months in the refrigerator if stored promptly after use.

  Related Links and Documents

  • Accutase Solution
• What is the difference between the PCR Mycoplasma Test Kit I/C (PK-CA91-1024) and PCR Mycoplasma Test Kit II (PK-CA20-700-20)?

  Both PCR Mycoplasma Test Kits are designed for conventional PCR but differ in the following points:

  • Format: The reagents are delivered lyophilized in Test Kit I/C, liquid (5x master mix) in Test Kit II.
  • Detection Spectrum: The spectrum of detected strains is slightly different: Test Kit II also detects e.g. M. pneumoniae and Spiroplasma citrii (which are both mentioned in the EUR. PHARM. as test/reference species) and a few others, which are however of no importance in cell cultures.
  • Size of internal controls:
    – PCR Mycoplasma Test Kit I/C ⇒ internal control: 479 bp, positive control: 270 bp
    – PCR Mycoplasma Test Kit II ⇒ internal control: 357 bp, positive control: 270 bp

  Related Links and Documents

  • PCR Mycoplasma Test Kit I/C
  • PCR Mycoplasma Test Kit II