Normal Human Dermal Fibroblasts (NHDF)

Primary Normal Human Dermal Fibroblasts isolated from the dermis of juvenile foreskin or adult skin.

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Primary Normal Human Dermal Fibroblasts (NHDF) are isolated form the dermis of juvenile foreskin or adult skin from different locations like the face, the breasts, the abdomen, and the thighs.

They can be used for wound healing studies and dermatological research to investigate diseases like scleroderma, fibrosarcoma, fibrosis, xeroderma pigmentosum, and histiocytoma. Moreover, fibroblasts are important for cancer research, tissue regeneration, and tissue engineering studies.

Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are available on request.

Available formats:

  • Cryopreserved: Cryogenic vial containing 500.000 viable cells.
  • Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
  • Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
Recommended Plating Density 3.500 – 7.000 cells per cm2
Passage After Thawing P2
Tested Markers CD90 positive
Guaranteed Population Doubling > 15
Guaranteed Population Doublings > 15
Figure 1. Normal Human Dermal Fibroblast Cell Culture in phase contrast
Figure 2. NHDF culture stained for CD90. Nuclei counterstained with DAPI.