Human Dermal Microvascular Endothelial Cells (HDMEC)

Primary Human Dermal Microvascular Endothelial Cells isolated from the dermis of juvenile foreskin and adult skin.

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Primary Human Dermal Microvascular Endothelial Cells (HDMEC) are isolated from the dermis of juvenile foreskin and adult skin (different locations). Since the dermis contains blood and lymphatic capillaries, HDMEC comprise Blood and Lymphatic Microvascular Endothelial Cells. Both have a common origin and can be identified by several markers.

In addition, HDMEC are available which are pre-screened for VEGF (vascular endothelial growth factor) response. VEGF is an important signaling protein involved in both vasculogenesis and angiogenesis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesis, cell migration, sprouting, and microvascular permeability.

Available formats:

  • Cryopreserved: Cryogenic vial containing 500.000 viable cells.
  • Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
  • Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
Recommended Plating Density 10.000 – 20.000 cells per cm2
Passage After Thawing P2
Tested Markers von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positive
Guaranteed Population Doubling > 15
Guaranteed Population Doublings > 15
Figure 1. Human Dermal Microvascular Endothelial Cell Culture in phase contrast
Figure 2. HDMEC culture stained for vWF (Factor VIII antigen). Nuclei counterstained with DAPI.
Figure 3.