Cancer Cell Culture
8 Application Notes
Depletion of benign cells from established cancer cell cultures with the PromoCell Primary Cancer Culture System D-AFCTraditional culture systems for cancer cells all share a lack of specificity for malignant cells. PromoCell's Primary Cancer Culture System is a reliable tool for depleting stromal cells, fibroblasts and all other types of non-cancerous cells.
Establishment of non-selective adherent primary cultures from dissociated samples of solid human tumors and xenograftsLearn more about serum-free, non-selective primary culture of cells derived from tumor samples.
Standardized Culture of Established Cancer Cell Lines under Serum-free and Xeno-free ConditionsUndefined media supplements have unwanted effects on cell cultures. Learn how to obtain more accurate and comparable results with PromoCell’s Cancer Cell Line Medium XF.
Selective In Vitro Culture of Primary Human Cancer Cells from Human Tumor SamplesIsolate tumor cells from patient samples and transform them into long-term cell cultures. Learn more about the Primary Cancer Culture System and how it compares to other established culture methods.
Extended Neurosphere Culture of Brain Tumor Stem Cells with the PromoCell 3D Tumorsphere Medium XFUsing 3D cultures of brain cancer cells as neurospheres in serum-free conditions maintains the properties of primary tumors. In vitro models with the 3D Tumorsphere Medium XF are an essential prerequisite for research aimed at identifying ways to eradicate brain tumor cancer stem cells.
Extended Mammosphere Culture of Human Breast Cancer CellsPromoCell 3D Tumorsphere Medium XF allows robust 3D culture even when using breast-cancer cell lines. Mammospheres are now regarded as highly valuable in vitro models for studies on tumor formation and metastasis.
Determining Tumorsphere Formation Efficiency (TFE) with the PromoCell 3D Tumorsphere Medium XFDetermine the tumorsphere formation efficiency using an assay to quantify the ability of single cells to form spheres. Our defined and xeno-free 3D Tumorsphere Medium enables you to cultivate cancer stem cells.
- Dendritic Cell Generation Media 1 Application Note
3 Application Notes
Research Note – Vascular Cell Culture under Physiological Oxygen Tensions – Implications for Cellular Redox HomeostasisIf the amount of reactive oxygen species exceeds the cells antioxidant capacity, your research subjects are stressed. Find out how you can shift your cell culture conditions and establish physiological conditions.
Endothelial Cell Tube Formation Assay (Angiogenesis Assay)Different processes lead to formation of new blood vessels in the human body. The in vitro tube formation assay allows researchers to study such mechanisms in cell culture.
Hematopoietic Progenitor Cells
1 Application Note
Expansion of Hematopoietic Progenitor CellsResearchers are facing difficulties during in vitro expansion of undifferentiated hematopoietic stem cells (HSC). PromoCell Hematopoietic Progenitor Cell (HPC) Expansion Medium DXF provides a highly optimized, chemically defined, and xeno-free culture system for human HSC.
3 Application Notes
Assay-ready Adherent Cultures of Fully Functional Human Primary Macrophages Using Cryopreserved CellsCryopreserved human macrophages are now available as a reliable source of standardized cells, and our ready-to-use format provides full experimental flexibility. With this easy-to-handle application, your macrophage research will be more efficient.
Convenient Large-Scale Differentiation of Fully Customized Monocyte-Derived Macrophages From Human PBMC In VitroPrimary human macrophages are difficult to isolate and do not proliferate in culture. PromoCell offers a complete system for easy and cost-efficient generation of pure monocyte-derived macrophages.
Mesenchymal Stem Cell Media
6 Application Notes
Metabolic Activity During Mesenchymal Stem cell (MSC) DifferentiationDetailed analysis of human stem cells offers the potential for the specific treatment of diseases by cell-based therapies. With an automated real-time analysis, we assess phenotypic changes during mesenchymal stem cell (MSC) differentiation.
Real-Time Analysis of Stem Cell DifferentiationHere we describe the real-time analysis of human stem cell differentiation using cell-based assays. This allows for quantification of cell viability, proliferation and apoptosis.
Neurogenic Differentiation and Analysis of Mesenchymal Stem Cells (MSC)Specific culture conditions allow for transdifferentiation of mesenchymal stem cells (MSC) into cells of non-mesenchymal origin such as neurons. To do so, specific culture conditions and stimuli are needed.
Osteogenic Differentiation and Analysis of Mesenchymal Stem Cells (MSC)Differentiate mesenchymal stem cells (MSC) into osteoblasts. Read our application note and find out more. The multipotency of these cells is one essential characteristic of MSC, according to the International Society for Cellular Therapy.
Chondrogenic Differentiation and Analysis of Mesenchymal Stem Cells (MSC)Managed in vitro differentiation of mesenchymal stem cells (MSC) into chondrocytes. Multipotent adult stem cells are important for various tissues of the musculoskeletal system.
- Osteoblasts 1 Application Note
PromoCell service does not end with product delivery. Along with our high quality products, we provide knowledge on using them optimally. Read our application notes for details and insights on how to work with our products in your lab.