Products

3D Tumorsphere Medium XF

(Cancer Stem Cell Medium) 


PromoCell introduces the next generation 3D Tumorsphere Medium. This medium has been designed to meet your requirements for the extended serial 3D tumorsphere culture. Our 3D Tumorsphere Medium supports the tumorsphere/mammosphere culture of the most commonly used cancer cell lines.

Setting a New Standard with Next Generation Tumorsphere Medium


Key Advantages:

  • Easy 3D tumorsphere culture system
  • Directly switch from 2D to 3D culture
  • Supports most commonly used cancer cell lines
  • Allows extended serial tumorsphere passages (>10)
  • High tumorsphere formation efficiency
  • Xeno-free and serum-free formulation
  • Ready to use 

 

TissueTested Cell LinesCell Lines Origin
BrainU-87 MGgrade IV glioblastoma / astrocytoma of the human brain
BreastMCF-7 pleural effusion of metastatic human breast adenocarcinoma
Breast  MDA-MB-231 pleural effusion of metastatic human breast adenocarcinoma (triple-negative)
ColonHT-29human colon adenocarcinoma
Connective tissueHT1080human fibrosarcoma
LiverHepG2hepatocellular carcinoma of the human liver
LungA-549human lung carcinoma
PancreasPanc-1epithelioid carcinoma of the human pancreatic duct
ProstateLNCaPlymph node metastasis of human prostate adenocarcinoma
SkinA-431epidermoid carcinoma of the human skin

3D Tumorsphere Medium XF with 
SupplementMix                       

List of cell types tested for serial passage with the PromoCell
3D Tumorsphere Medium XF.


References, Questions & Answers

Product Name Size Catalog Number Information Prices
3D Tumorsphere Medium XF 250 ml C-28070 click to select country
3D Tumorsphere Medium XF, phenol red-free 250 ml C-28075 click to select country


Related products

     PromoCell's 3D Tumorsphere Medium XF has been designed to meet your requirements
     for the extended serial routine-culture of tumorspheres/mammospheres.

 

The 3D Tumorsphere Medium XF Culture System

        Proliferation and tumorsphere formation efficiency (TFE) of MCF-7 cells during serial passage in 3D tumorsphere
        culture using the PromoCell 3D Tumorsphere Medium XF.
        Robust proliferation was maintained during the culture, which was discontinued after passage 10 with no sign of
        growth rate inhibition. Serial passage in the 3D Tumorsphere Medium XF resulted in significant increase of TFE from
        2% in P1 to 14% in P4, and 28% in P9, respectively.

        Examples of proliferation curves of various established cancer cell
        lines expanded by serial passage of 3D tumorsphere cultures using
        the PromoCell 3D Tumorsphere Medium XF.

 

Passage 10 serial tumosphere culture
of HT1080 fibrosarcoma cells using
our 3D Tumorsphere Medium XF.

 

 

Passage 10 serial tumosphere culture
of MCF-7 mammary carcinoma cells
using our 3D Tumorsphere Medium XF.

 

 

3D Tumorsphere Medium XF
culture of HT-29 colon carcinoma cells.
The spheres tend to loosely attach to
the culture vessel surface and can be
easily removed for passage by flushing
with a pipet.