Primary Cancer Culture System
A complete cell culture solution designed for the selective culture of malignant cells derived from primary tumors or patient-derived xenografts.
PromoCell’s Primary Cancer Culture System is a standardized, defined and animal component-free (D-ACF) system for the isolation and culture of human primary tumor cells. The medium was designed to be the first universally applicable in vitro cell culture solution for the isolation and long-term culture of primary human malignancies, e.g. from patient tumor samples or patient-derived xenografts (PDX).
Due to precise stromal control, prolonged culture allows for functional selection of malignant cells. This principle enables access to an enriched population of primary cancer cells. The selection process dispenses with the use of toxic agents. Since on the cellular level malignancy itself is the only selection criterion, the cell diversity of the cancerous subpopulations of the original tumor is preserved.
- Perform up to five primary tumor isolations with one medium kit
- Successfully prevent stroma overgrowth when isolating fresh tumor samples
- Establish a culture of pure malignant cells within 4-6 weeks
- Culture PDX tumor models in vitro
- Defined and animal component-free formulation
- Primary Cancer Cell Medium D-ACF, 250ml
- SupplementMix for 250ml
- NCCD surface treatment reagent, 2ml
The Primary Cancer Culture System can also be used for other applications, e.g. enriching cell lines or depleting of stromal cells and other non-cancerous cells form established primary cancer cell cultures.
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Depletion of benign cells from established cancer cell cultures with the PromoCell Primary Cancer Culture System D-AFCTraditional culture systems for cancer cells all share a lack of specificity for malignant cells. PromoCell's Primary Cancer Culture System is a reliable tool for depleting stromal cells, fibroblasts and all other types of non-cancerous cells.
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