Primary Cancer Culture System
A complete cell culture solution designed for the selective culture of malignant cells derived from primary tumors or patient-derived xenografts.
PromoCell’s Primary Cancer Culture System is a standardized, defined and animal component-free (D-ACF) system for the isolation and culture of human primary tumor cells. The medium was designed to be the first universally applicable in vitro cell culture solution for the isolation and long-term culture of primary human malignancies, e.g. from patient tumor samples or patient-derived xenografts (PDX).
Due to precise stromal control, prolonged culture allows for functional selection of malignant cells. This principle enables access to an enriched population of primary cancer cells. The selection process dispenses with the use of toxic agents. Since on the cellular level malignancy itself is the only selection criterion, the cell diversity of the cancerous subpopulations of the original tumor is preserved.
- Perform up to five primary tumor isolations with one medium kit
- Successfully prevent stroma overgrowth when isolating fresh tumor samples
- Establish a culture of pure malignant cells within 4-6 weeks
- Culture PDX tumor models in vitro
- Defined and animal component-free formulation
- Primary Cancer Cell Medium D-ACF, 250ml
- SupplementMix for 250ml
- NCCD surface treatment reagent, 2ml
The Primary Cancer Culture System can also be used for other applications, e.g. enriching cell lines or depleting of stromal cells and other non-cancerous cells form established primary cancer cell cultures.
Technical Library (1)
Does the PromoCell 3D Tumorsphere Medium XF (C-28070) maintain the stem cell character of cancer stem cells?
During our quality control we do not determine the presence or maintenance of respective stem cell markers in the tumorspheres. We use a functional approach instead, by culturing the tumorspheres over serial passages in C-28070. Tumorsphere formation requires the biological features of Anoikis resistance and self-renewal and therefore indicates the presence of cancer stem cells (CSC)/cancer initiating cells (CIC) in the spheres. 3D tumorsphere culture thus allows scientists to study the biology of CSCs without any background knowledge on CSC markers.
Related Links and Documents
- Does the PromoCell 3D Tumorsphere Medium XF (C-28070) maintain the stem cell character of cancer stem cells?
Application Notes (4)
Selective In Vitro Culture of Primary Human Cancer Cells from Human Tumor SamplesIsolate tumor cells from patient samples and transform them into long-term cell cultures. Learn more about the Primary Cancer Culture System and how it compares to other established culture methods.
Research Note – Vascular Cell Culture under Physiological Oxygen Tensions – Implications for Cellular Redox HomeostasisIf the amount of reactive oxygen species exceeds the cells antioxidant capacity, your research subjects are stressed. Find out how you can shift your cell culture conditions and establish physiological conditions.
Endothelial Cell Tube Formation Assay (Angiogenesis Assay)Different processes lead to formation of new blood vessels in the human body. The in vitro tube formation assay allows researchers to study such mechanisms in cell culture.
Convenient Large-Scale Differentiation of Fully Customized Monocyte-Derived Macrophages From Human PBMC In VitroPrimary human macrophages are difficult to isolate and do not proliferate in culture. PromoCell offers a complete system for easy and cost-efficient generation of pure monocyte-derived macrophages.
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