PromoCell’s Primary Cancer Culture System is a standardized, defined and animal component-free (D-ACF) system for the isolation and culture of human primary tumor cells. The medium was designed to be the first universally applicable in vitro cell culture solution for the isolation and long-term culture of primary human malignancies, e.g. from patient tumor samples or patient-derived xenografts (PDX).
Due to precise stromal control, prolonged culture allows for functional selection of malignant cells. This principle enables access to an enriched population of primary cancer cells. The selection process dispenses with the use of toxic agents. Since on the cellular level malignancy itself is the only selection criterion, the cell diversity of the cancerous subpopulations of the original tumor is preserved.
Perform up to five primary tumor isolations with one medium kit
Successfully prevent stroma overgrowth when isolating fresh tumor samples
Establish a culture of pure malignant cells within 4-6 weeks
The Primary Cancer Culture System can also be used for other applications, e.g. enriching cell lines or depleting of stromal cells and other non-cancerous cells form established primary cancer cell cultures.
Self-destruction of the body: Cancer turns normal body cells into their malignant counterpart that wants to keep on replicating by any means. Understanding the individual disease offers the potential for more effective therapies with fewer side effects. The new PromoCell Primary Cancer Culture System allows researchers to generate high-fidelity in vitro models of primary tumors….
Many scientists working in oncology focus on cancer stem cells (CSCs). Targeting these cells specifically could improve cancer therapy significantly. Because CSCs rely heavily on their environment, three-dimensional cultivation offers the unique opportunity to better understand this interaction. We talked to two scientists working in this field of cancer research.
In the human body,…
Antibiotics are routinely used in cell cultures to prevent bacterial infections. But there are side effects: Studies show that they impair cell growth and differentiation. With good laboratory practice, the use of antibiotics is unnecessary.
A cell culture is a world of its own. The culture dish contains nutrients within the media that cells need to thrive and prosper….
Oxygen has two faces: While it’s a life-saving gas essential for humans and animals, excessive generation of reactive oxygen species damages cells and promotes senescence. This duality is the research focus of Professor Giovanni E. Mann of King’s College London. Prof. Mann and his colleagues are currently investigating redox signaling in vascular cells in culture….
What seems basic to cell culturing is pure stress for primary cells: thawing, subcultivation and freezing. Even seasoned scientists tend to overlook this aspect, and manipulate their cultures less carefully than they should. Unhealthy, or even dead cells are the sad consequence. Here, you can benefit from an expert’s tips in handling primary cells,…
Selected as the 2013 “Breakthrough of the Year” by Science magazine, cancer immunotherapy shows a completely new way to treat malignancies by using the body’s own immune system, and not by targeting the tumor itself. This cutting-edge area of research and therapy has shown very promising results. However, further investigation is needed to turn immunotherapy into a cancer therapy that can be broadly applied….
Researchers are increasingly using 3D cell cultures to study the skin’s features. Three-dimensional skin models offer the potential to study various diseases and test compounds in vitro. Dr. Florian Groeber-Becker from the Fraunhofer Institute for Silicate Research ISC in Wurzburg has developed a highly sophisticated skin model.
It is the first thing people see when they look at us….
Human umbilical vein cells (HUVECs) are easy to access, so they have been the major source of primary endothelial cells (ECs) for years. But to study adult vascular pathology, bring your endothelial research to the next level – by choosing adult ECs that more accurately resemble in vitro conditions….
Cells that feed other cells: The endothelium is a layer of cells that line blood vessels, and it is responsible for many tasks, including the transport of essential nutrients. Human umbilical vein endothelial cells (HUVECs) provide an in-vitro model for studying physiological and pathological processes of the vasculature.
“I’ll never forget the first time I added cancer cells to a HUVECs monolayer and watched them interact….
One cell type can fight disease, help form blood vessels, regulate cell stability— and more. Macrophages are true masters of multitasking. In our body they are the first line of defense against invading bacteria, fungi and viruses. But they are not only a key element of our immune defense; they can also play a role in destroying tissue,…