Primary Cancer Culture System

A complete cell culture solution designed for the selective culture of malignant cells derived from primary tumors or patient-derived xenografts.

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Primary Cancer Culture System
  250 ml
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PromoCell’s Primary Cancer Culture System is a standardized, defined and animal component-free (D-ACF) system for the isolation and culture of human primary tumor cells. The medium was designed to be the first universally applicable in vitro cell culture solution for the isolation and long-term culture of primary human malignancies, e.g. from patient tumor samples or patient-derived xenografts (PDX).

Due to precise stromal control, prolonged culture allows for functional selection of malignant cells. This principle enables access to an enriched population of primary cancer cells. The selection process dispenses with the use of toxic agents. Since on the cellular level malignancy itself is the only selection criterion, the cell diversity of the cancerous subpopulations of the original tumor is preserved.

Key features:

  • Perform up to five primary tumor isolations with one medium kit
  • Successfully prevent stroma overgrowth when isolating fresh tumor samples
  • Establish a culture of pure malignant cells within 4-6 weeks
  • Culture PDX tumor models in vitro
  • Defined and animal component-free formulation


The Primary Cancer Culture System can also be used for other applications, e.g. enriching cell lines or depleting of stromal cells and other non-cancerous cells form established primary cancer cell cultures.

Figure 1. Overview of the selective isolation of primary cancer cells from human tumor samples using the Primary Cancer Culture System. The PromoCell system makes it possible to reliably deplete non-malignant cells from the cell culture while supporting the maintenance of cancer cells and malignant cell heterogeneity. The optional establishment of cancer cell lines is indicated.
Figure 2. Primary culture derived from a squamous cell carcinoma featuring an adherent growth pattern. The upper right inset reveals cellular heterogeneity at a greater magnification.
Figure 3. Primary culture derived from a low-grade small cell lung cancer featuring a suspension growth pattern with 3D sphere formation.