NCCD Reagent is part of PromoCell’s Primary Cancer Culture System and is crucial for the pre-treatment of the culture vessel.
Being a part of PromoCell’s Primary Cancer Culture System the NCCD Reagent is intended exclusively for use in combination with the Primary Cancer Cell Culture Medium D-ACF. The NCCD Reagent allows for the culture vessel surface pre-treatment which is critical for the functionality of the Primary Cancer Cell Culture System.
The NCCD Reagent is provided as a 20x stock and must be diluted to a working concentration before use. One 2 ml vial of NCCD Reagent yields 40 ml of ready-to-use working solution sufficient to treat 400 cm2 of culture surface, e.g. seven complete 6-well plates.
Technical Library (1)
Does the PromoCell 3D Tumorsphere Medium XF (C-28070) maintain the stem cell character of cancer stem cells?
During our quality control we do not determine the presence or maintenance of respective stem cell markers in the tumorspheres. We use a functional approach instead, by culturing the tumorspheres over serial passages in C-28070. Tumorsphere formation requires the biological features of Anoikis resistance and self-renewal and therefore indicates the presence of cancer stem cells (CSC)/cancer initiating cells (CIC) in the spheres. 3D tumorsphere culture thus allows scientists to study the biology of CSCs without any background knowledge on CSC markers.
Related Links and Documents
- Does the PromoCell 3D Tumorsphere Medium XF (C-28070) maintain the stem cell character of cancer stem cells?
Application Notes (4)
Selective In Vitro Culture of Primary Human Cancer Cells from Human Tumor SamplesIsolate tumor cells from patient samples and transform them into long-term cell cultures. Learn more about the Primary Cancer Culture System and how it compares to other established culture methods.
Research Note – Vascular Cell Culture under Physiological Oxygen Tensions – Implications for Cellular Redox HomeostasisIf the amount of reactive oxygen species exceeds the cells antioxidant capacity, your research subjects are stressed. Find out how you can shift your cell culture conditions and establish physiological conditions.
Endothelial Cell Tube Formation Assay (Angiogenesis Assay)Different processes lead to formation of new blood vessels in the human body. The in vitro tube formation assay allows researchers to study such mechanisms in cell culture.
Convenient Large-Scale Differentiation of Fully Customized Monocyte-Derived Macrophages From Human PBMC In VitroPrimary human macrophages are difficult to isolate and do not proliferate in culture. PromoCell offers a complete system for easy and cost-efficient generation of pure monocyte-derived macrophages.
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