Keratinocyte Growth Medium 2

Serum-free cell culture medium for keratinocytes from the epidermis.

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PromoCell Keratinocyte Growth Medium 2 is a serum-free medium developed for the cultivation of epidermal keratinocytes without feeder cells.

The medium is optimized for primary human cells but can also be used for horse and porcine keratinocytes, as well as mouse and rat keratinocytes after adapting the CaCl2 concentration. In addition, it is suited for rat corneal keratinocytes.


  • Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix
  • Growth Medium Kit: Includes Basal Medium and SupplementPack
  • SupplementMix: Contains all media supplements premixed in one vial
  • SupplementPack: Contains all media supplements as individual vials
Media supplement Final supplement concentration
Bovine Pituitary Extract 0.004 ml / ml
Epidermal Growth Factor (recombinant human) 0.125 ng / ml
Insulin (recombinant human) 5 μg / ml
Hydrocortisone 0.33 μg / ml
Epinephrine 0.39 μg / ml
Transferrin (recombinant human) 10 μg / ml
CaCl2 0.06 mM

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