Human Umbilical Vein Endothelial Cells (HUVEC)

Primary Human Umbilical Vein Endothelial Cells isolated from the vein of the umbilical cord of single, pooled or pre-screened donors.

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Selected product Human Umbilical Vein Endothelial Cells (HUVEC)

single donor, cryopreserved 500,000 cells

single donor, proliferating 500,000 cells

pooled donors, cryopreserved 500,000 cells

pooled donors, proliferating 500,000 cells

pre-screened single donor, cryopreserved 500,000 cells

pre-screened single donor, proliferating 500,000 cells

single donor, cell pellet in RNAlater 1,000,000 cells

pooled donors, cell pellet in RNAlater 1,000,000 cells

pre-screened single donor, cell pellet in RNAlater 1,000,000 cells

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Primary Human Umbilical Vein Endothelial Cells (HUVEC) are isolated from the vein of the umbilical cord and are commonly used for physiological and pharmacological investigations, such as macromolecule transport, blood coagulation, angiogenesis, and fibrinolysis.

PromoCell offers HUVEC isolated in standard Endothelial Cell Growth Medium. The cells are supplied either from single donors or from pooled donors (from up to four different umbilical cords).
(Human Umbilical Artery Endothelial Cells (HUAEC) from the same donor are available on request.)

In addition, HUVEC are available which are pre-screened for VEGF (vascular endothelial growth factor) response. VEGF is an important signaling protein involved in both vasculogenesis and angiogenesis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesis, cell migration, sprouting, and microvascular permeability.

NEW: Our HUVEC are now also available from HLA-typed donors.

Available formats:

  • Cryopreserved: Cryogenic vial containing 500.000 viable cells.
  • Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
  • Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
Recommended plating density 5,000 – 10,000 cells per cm2
Passage After Thawing P1
Tested Markers CD31 positive, Dil-Ac-LDL uptake positive
Guaranteed Population Doubling > 15
Figure 1. Human Umbilical Vein Endothelial Cell Culture in phase contrast.
Figure 1. Human Umbilical Vein Endothelial Cell Culture in phase contrast.
Figure 2. HUVEC culture stained for CD31 antigen, Nuclei counterstained with DAPI.
Figure 2. HUVEC culture stained for CD31 antigen, Nuclei counterstained with DAPI.
Figure 3. HUVEC culture stained for vWF (Factor VIII antigen). Nuclei counterstained with DAPI.
Figure 3. HUVEC culture stained for vWF (Factor VIII antigen). Nuclei counterstained with DAPI.

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