Human Umbilical Artery Endothelial Cells (HUAEC)
Primary Human Umbilical Endothelial Cells isolated from the arteries of the umbilical cord.
Primary Human Umbilical Artery Endothelial Cells (HUAEC) are isolated from the arteries of the umbilical cord and are often used for in vitro studies on atherosclerosis but also for endothelial cell-dependent lymphocyte activation and chemoattractant activity experiments.
(Human Umbilical Vein Endothelial Cells (HUVEC) from the same donor are available on request).
NEW: Our HUAEC are now also available from HLA-typed donors.
- Cryopreserved: Cryogenic vial containing 500.000 viable cells.
- Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
- Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
|Recommended plating density||5,000 – 10,000 cells per cm2|
|Passage After Thawing||P1|
|Tested Markers||von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positive|
|Guaranteed Population Doubling||> 15|
Technical Library (3)
Which medium does PromoCell recommend for endothelial cell transfection ?
There are several factors that can influence successful transfections, e.g. viability and density of the cells, choice of the transfection reagent, quality and type of the transfected molecules (plasmids, siRNA, oligonucleotides), as well as the culture medium and supplements used.
When using PromoCell Endothelial Cell Growth Media for cell transfection, please follow the instructions below:
Heparin, which is included in our Endothelial Cell Growth Medium (C-22010/C-22110), Medium 2 (C-22011/C-22111) and Medium MV (C-22020/C-22120) may reduce the transfection efficiency. We therefore recommend to use our heparin-free Endothelial Cell Growth Medium MV2 (C-22022/C-22121). Alternatively, you may use Endothelial Cell Growth Medium Kit (C-22110), Medium 2 Kit (C-22111), or Medium MV Kit (C-22120) without adding the heparin supplement to the Basal Medium.
Please note: Before and after transfection, the cells should be cultured in complete Growth Medium including heparin to ensure optimal growth.
Related Links and Documents
Which medium do PromoCell recommend to use on HUVEC – Endothelial Cell Growth Medium or Endothelial Cell Growth Medium 2?
The standard medium for isolation and propagation of our HUVEC, HUAEC, HPAEC, and HSaVEC is Endothelial Cell Growth Medium (C-22010). It contains ECGS, an extract from bovine hypothalamus which has mitogenic effects on endothelial cell proliferation. Scientists who prefer a more defined Growth Medium can use Endothelial Cell Growth Medium 2 (C-22011). In this medium, ECGS is replaced by VEGF, IGF, and additional bFGF and EGF to stimulate endothelial cell growth.
Related Links and Documents
At what passage do PromoCell supply the cell pellets?
The majority of PromoCell cell pellets are prepared 1 passage after thawing the cryopreserved cells. Examples: HUVEC and HUAEC correspond to P1 after thawing; therefore the pellets are frozen in P2. SMC or keratinocytes or epithelial cells correspond to P2 after thawing; therefore the pellets are frozen in P3. In contrast, our blood cells are cryopreserved directly after cell isolation. These pellets are prepared after thawing the cryovials with no further cultivation step.
Related Links and Documents
- Which medium does PromoCell recommend for endothelial cell transfection ?
Application Notes (0)
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Reference Literature (15)
Efficient and robust differentiation of endothelial cells from human induced pluripotent stem cells via lineage control with VEGF and cyclic AMP
Ikuno et al.; PLoS One. 2017 Mar 13;12(3):e0173271
Extracellular antibody drug conjugates exploiting the proximity of two proteins
Marshall et al.; Mol Ther. 2016 Oct;24(10):1760-1770
A role for GPR55 in human placental venous endothelial cells
Kremshofer et al.; Histochem Cell Biol. 2015 Jul;144(1):49-58
Piezo1 integration of vascular architecture with physiological force
Li et al.; Nature. 2014 Nov 13;515(7526):279-82
Cyclosporine triggers endoplasmic reticulum stress in endothelial cells: a role for endothelial phenotypic changes and death
Bouvier et al.; Am J Physiol Renal Physiol. 2009 Jan;296(1):F160-9
The extracellular matrix is a novel attribute of endothelial progenitors and of hypoxic mature endothelial cells
Kusuma et al.; FASEB J. 2012 Dec;26(12):4925-36
GATA6 promotes angiogenic function and survival in endothelial cells by suppression of autocrine transforming growth factor beta/activin receptor-like kinase 5 signaling
Froese et al.; J Biol Chem. 2011 Feb 18;286(7):5680-90
Pulsatile shear and Gja5 modulate arterial identity and remodeling events during flow-driven arteriogenesis
Buschmann et al.; Development. 2010 Jul;137(13):2187-96
The Tie-2 ligand Angiopoietin-2 is stored in and rapidly released upon stimulation from endothelial cell Weibel-Palade bodies
Fiedler et al.; Blood. 2004 Jun 1;103(11):4150-6
Sodium fluoride induces podosome formation in endothelial cells
Tatin et al.; Biol Cell. 2010 Jul 6;102(9):489-98
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Endothelial Cell TypesWant to know more about our portfolio of endothelial cells? Take a look at our poster for an overview of our large-vessel and microvascular endothelial cells, and where they are located in the body.
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