PromoCell’s cryopreserved human monocyte-derived macrophages (hMDM) are convenient and easy-to-handle. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks. Optionally, user-customizable activation of the cells can be performed.
Cryopreserved human macrophages are produced from monocytes in PromoCell’s well-proven M1 Macrophage Generation Media DXF and are available as non-activated, fully qualified M1- (GM-CSF) polarized cells. After 9-10 days of differentiation, the M1 macrophages are cryopreserved using PromoCells’s proprietary, defined and animal-free freezing medium, Cryo-SFM. Each cryo vial contains more than 1.5 million or 5 million viable cells after thawing, respectively.
NEW: Our M1 Macrophages are now also available from HLA-typed donors.
|Recommended plating density||100,000 cells per cm2|
|Tested Markers||CD80 positive, CD68 positive|