Human M1 Macrophages (GM-CSF) monocyte-derived

Monocyte-derived, polarized, non-activated, primary human macrophages. Polarization in M1-Macrophage Generation Medium DXF (incl. GM-CSF).

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PromoCell’s cryopreserved human monocyte-derived macrophages (hMDM) are convenient and easy-to-handle. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks. Optionally, user-customizable activation of the cells can be performed.

Cryopreserved human macrophages are produced from monocytes in PromoCell’s well-proven M1 Macrophage Generation Media DXF and are available as non-activated, fully qualified M1- (GM-CSF) polarized cells. After 9-10 days of differentiation, the M1 macrophages are cryopreserved using PromoCells’s proprietary, defined and animal-free freezing medium, Cryo-SFM. Each cryo vial contains more than 1.5 million or 5 million viable cells after thawing, respectively.

Recommended plating density 100,000 cells per cm2
Tested Markers CD80 positive, CD68 positive
Figure 1. Human Macrophage Cell Culture in phase contrast
Figure 1. Human Macrophage Cell Culture in phase contrast

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