Human Dermal Lymphatic Endothelial Cells (HDLEC)

Primary Human Dermal Lymphatic Endothelial Cells isolated from different sources (e.g. juvenila foreskin). CD31 positive, Podoplanin positive.

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Primary Human Dermal Lymphatic Endothelial Cells (HDLEC) are a subpopulation of the Human Dermal Endothelial Cells. They are isolated from the dermis of juvenile foreskin and adult skin (different locations) from a single donor.
(Human Dermal Blood Endothelial Cells (HDBEC) from the same donor are available on request.)

The cells are routinely analyzed by flow cytometric analysis and immunofluorescent staining:
> 95% of the cells are CD31 positive and podoplanin positive. In addition, the cells stain positive for Prox-1.

Lymphatic vessels transport excess fluids from tissues to the circulatory system and are a major component of the immune system. The transported fluid is nearly cell free. The vessels are highly permeable, because they lack a continuous basal membrane. Lymphatic endothelial cells are involved in pathological alterations of the lymphatic system. During tumor lymphangiogenesis, the lymphatic endothelial cells build new vessels that infiltrate tumors, attract tumor cells, and induce tumor cell metastasis.

Available formats:

  • Cryopreserved: Cryogenic vial containing 500.000 viable cells.
  • Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
  • Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
Recommended Plating Density 10.000 – 20.000 cells per cm2
Passage After Thawing P2
Tested Markers Podoplanin negative, CD31 positive
Guaranteed Population Doubling > 15
Guaranteed Population Doublings > 15
Figure 1.
Figure 2. Flow cytometric analyis of PromoCell HDLEC. 99% of the cells are CD31 positive.
Figure 3. Flow cytometric analysis of PromoCell HDLEC. 96% of the cells are podoplanin positive.