Human Dermal Blood Endothelial Cells (HDBEC)
Primary Human Dermal Blood Endothelial Cells isolated from different sources (e.g. juvenile foreskin). CD31 positive, Podoplanin negative.
Primary Human Dermal Blood Endothelial Cells (HDBEC) are a subpopulation of the Human Dermal Endothelial Cells. They are isolated from the dermis of juvenile foreskin and adult skin (different locations) from a single donor.
(Human Dermal Lymphatic Endothelial Cells (HDLEC) from the same donor are available on request.)
The cells are routinely analyzed by flow cytometric analysis and immunofluorescent staining: they are > 95% CD31 positive and stain positive for von Willebrand factor. In addition, they are > 90% negative for podoplanin and stain negative for smooth muscle specific alpha-actin.
Blood Endothelial Cells have a key function in physiological processes like vessel tonus, capillary permeability, blood coagulation, fibrolysis, and angiogenesis. They are also useful for disease studies of atherosclerosis, tumor genesis, and thrombosis.
- Cryopreserved: Cryogenic vial containing 500.000 viable cells.
- Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
- Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
|Recommended Plating Density||10.000 – 20.000 cells per cm2|
|Passage After Thawing||P2|
|Tested Markers||Podoplanin negative, CD31 positive|
|Guaranteed Population Doubling||> 15|
|Guaranteed Population Doublings||> 15|
Technical Library (2)
What are the characteristic markers of HDLEC (C-12216, C-12217) and HDBEC (C-12211, C-12225)?
- HDLEC and HDBEC both express the typical endothelial cell marker CD31 (= PECAM-1).
- HDLEC cultures are additionally tested positive for podoplanin, a transmembrane glycoprotein involved in lymphatic vessel formation, whereas HDBECs are podoplanin-negative.
Related Links and Documents
Does PromoCell determine the percentage of HDLEC in its HDMEC lots?
No, we don’t determine the ratio of HDLEC and HDBEC in our HDMEC lots. From our experience, the percentage of HDLEC is highly lot-dependent and can vary between 5 and 60%.
Related Links and Documents
- What are the characteristic markers of HDLEC (C-12216, C-12217) and HDBEC (C-12211, C-12225)?
Application Notes (0)
Sorry, there are no results for "HDBEC" in Application Notes.
Reference Literature (21)
Endocrine regulation of lymphocyte trafficking in vitro
Apta et al.; Methods Mol Biol. 2017;1591:101-119
Monocyte subsets coregulate inflammatory responses by integrated signaling through TNF and IL-6 at the endothelial cell interface
Chimen et al.; J Immunol. 2017 Apr 1;198(7):2834-2843
Leukocytes breach endothelial barriers by insertion of nuclear lobes and disassembly of endothelial actin filaments
Barzilai et al.; Cell Rep. 2017 Jan 17;18(3):685-699
Carbohydrate-binding protein CLEC14A regulates VEGFR-2- and VEGFR-3-dependent signals during angiogenesis and lymphangiogenesis
Lee at al.; J Clin Invest. 2017 Feb 1;127(2):457-471
Efficacy of AdipoDren in reducing interleukin-1-induced lymphatic endothelial hyperpermeability
Ciccone et al.; J Vasc Res. 2016;53(5-6):255-268
Morphological and molecular characterization of human dermal lymphatic collectors
Hasselhof et al.; PLoS One. 2016 Oct 20;11(10):e0164964
Simvastatin pretreatment reduces caspase-9 and RIPK1 protein activity in rat cardiac allograft ischemia-reperfusion
Tuuminen et al.; Transpl Immunol. 2016 Jul;37:40-5
Short-term ultraviolet A irradiation leads to dysfunction of the limbal niche cells and an antilymphangiogenic and anti-inflammatory micromilieu
Notara et al.; Invest Ophthalmol Vis Sci. 2016 Mar 1;57(3):928-39
Comparing the hem- and lymphangiogenic profile of conjunctival and uveal melanoma cell lines
Refaian et al.; Invest Ophthalmol Vis Sci. 2015 Aug 1;56(9):5691-7
Heterogeneity in the locomotory behavior of human monocyte subsets over human vascular endothelium in vitro
Collison et al.; J Immunol. 2015 Aug 1;195(3):1162-70
Sorry, there are no results for "HDBEC" in Posters.
Sorry, there are no results for "HDBEC" in Brochures.
Sorry, there are no results for "HDBEC" in FAQs.
Sorry, there are no results for "HDBEC" in Blog.