Human Dermal Blood Endothelial Cells (HDBEC)

Primary Human Dermal Blood Endothelial Cells isolated from different sources (e.g. juvenile foreskin). CD31 positive, Podoplanin negative.

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Primary Human Dermal Blood Endothelial Cells (HDBEC) are a subpopulation of the Human Dermal Endothelial Cells. They are isolated from the dermis of juvenile foreskin and adult skin (different locations) from a single donor.
(Human Dermal Lymphatic Endothelial Cells (HDLEC) from the same donor are available on request.)

The cells are routinely analyzed by flow cytometric analysis and immunofluorescent staining: they are > 95% CD31 positive and stain positive for von Willebrand factor. In addition, they are > 90% negative for podoplanin and stain negative for smooth muscle specific alpha-actin.

Blood Endothelial Cells have a key function in physiological processes like vessel tonus, capillary permeability, blood coagulation, fibrolysis, and angiogenesis. They are also useful for disease studies of atherosclerosis, tumor genesis, and thrombosis.

Available formats:

  • Cryopreserved: Cryogenic vial containing 500.000 viable cells.
  • Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
  • Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
Recommended Plating Density 10.000 – 20.000 cells per cm2
Passage After Thawing P2
Tested Markers Podoplanin negative, CD31 positive
Guaranteed Population Doubling > 15
Guaranteed Population Doublings > 15
Figure 1. Flow cytometric analyis of PromoCell HDBEC. 98% of the cells are CD31 positive.
Figure 2. Flow cytometric analysis of PromoCell HDBEC. 91% of the cells are podoplanin negative.