Human Dermal Blood Endothelial Cells (HDBEC)

Primary Human Dermal Blood Endothelial Cells isolated from different sources (e.g. juvenile foreskin). CD31 positive, Podoplanin negative.

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Primary Human Dermal Blood Endothelial Cells (HDBEC) are a subpopulation of the Human Dermal Endothelial Cells. They are isolated from the dermis of juvenile foreskin and adult skin (different locations) from a single donor.
(Human Dermal Lymphatic Endothelial Cells (HDLEC) from the same donor are available on request.)

The cells are routinely analyzed by flow cytometric analysis and immunofluorescent staining: they are > 95% CD31 positive and stain positive for von Willebrand factor. In addition, they are > 90% negative for podoplanin and stain negative for smooth muscle specific alpha-actin.

Blood Endothelial Cells have a key function in physiological processes like vessel tonus, capillary permeability, blood coagulation, fibrolysis, and angiogenesis. They are also useful for disease studies of atherosclerosis, tumor genesis, and thrombosis.

Recommended Plating Density 10.000 – 20.000 cells per cm2
Passage After Thawing P2
Tested Markers Podoplanin negative, CD31 positive
Guaranteed Population Doublings > 15
Figure 1. Flow cytometric analyis of PromoCell HDBEC. 98% of the cells are CD31 positive.
Figure 2. Flow cytometric analysis of PromoCell HDBEC. 91% of the cells are podoplanin negative.

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