Human Dermal Blood Endothelial Cells (HDBEC)
Primary Human Dermal Blood Endothelial Cells isolated from different sources (e.g. juvenile foreskin). CD31 positive, Podoplanin negative.
Primary Human Dermal Blood Endothelial Cells (HDBEC) are a subpopulation of the Human Dermal Endothelial Cells. They are isolated from the dermis of juvenile foreskin and adult skin (different locations) from a single donor.
(Human Dermal Lymphatic Endothelial Cells (HDLEC) from the same donor are available on request.)
The cells are routinely analyzed by flow cytometric analysis and immunofluorescent staining: they are > 95% CD31 positive and stain positive for von Willebrand factor. In addition, they are > 90% negative for podoplanin and stain negative for smooth muscle specific alpha-actin.
Blood Endothelial Cells have a key function in physiological processes like vessel tonus, capillary permeability, blood coagulation, fibrolysis, and angiogenesis. They are also useful for disease studies of atherosclerosis, tumor genesis, and thrombosis.
NEW: Our HDBEC are now also available from HLA-typed donors.
- Cryopreserved: Cryogenic vial containing 500.000 viable cells.
- Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
- Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
|Recommended plating density||10,000 – 20,000 cells per cm2|
|Passage After Thawing||P2|
|Tested Markers||Podoplanin negative, CD31 positive|
|Guaranteed Population Doubling||> 15|
Technical Library (2)
What are the characteristic markers of HDLEC (C-12216, C-12217) and HDBEC (C-12211, C-12225)?
- HDLEC and HDBEC both express the typical endothelial cell marker CD31 (= PECAM-1).
- HDLEC cultures are additionally tested positive for podoplanin, a transmembrane glycoprotein involved in lymphatic vessel formation, whereas HDBECs are podoplanin-negative.
Related Links and Documents
Does PromoCell determine the percentage of HDLEC in its HDMEC lots?
No, we don’t determine the ratio of HDLEC and HDBEC in our HDMEC lots. From our experience, the percentage of HDLEC is highly lot-dependent and can vary between 5 and 60%.
Related Links and Documents
- What are the characteristic markers of HDLEC (C-12216, C-12217) and HDBEC (C-12211, C-12225)?
Application Notes (0)
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