Human Coronary Artery Endothelial Cells (HCAEC)

Primary Human Endothelial Cells isolated from the human coronary arteries from single donors.

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Primary Human Coronary Artery Endothelial Cells (HCAEC) are isolated from the coronary arteries (the right and the left coronary artery, including the anterior descending and the circumflex branches) from a single donor. They are crucially involved in the regulation of coronary blood flow and cardiac functions and are consequently useful for in vitro studies of diseases such as thrombosis, atherosclerosis, and hypertension. HCAEC are also suitable for studying functional vaso-dilators, such as prostacyclin, bradykinin, or nitric oxide.

Available formats:

  • Cryopreserved: Cryogenic vial containing 500.000 viable cells.
  • Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
  • Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
Recommended plating density 5,000 – 10,000 cells per cm2
Passage After Thawing P3
Tested Markers von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positive
Guaranteed Population Doubling > 15
Figure 1. Location of the coronary arteries.
Figure 1. Location of the coronary arteries.

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