Primary Human Coronary Artery Endothelial Cells (HCAEC) are isolated from the coronary arteries (the right and the left coronary artery, including the anterior descending and the circumflex branches) from a single donor. They are crucially involved in the regulation of coronary blood flow and cardiac functions and are consequently useful for in vitro studies of diseases such as thrombosis, atherosclerosis, and hypertension. HCAEC are also suitable for studying functional vaso-dilators, such as prostacyclin, bradykinin, or nitric oxide.
- Cryopreserved: Cryogenic vial containing 500.000 viable cells.
- Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
- Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
|Recommended plating density||5,000 – 10,000 cells per cm2|
|Passage After Thawing||P3|
|Tested Markers||von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positive|
|Guaranteed Population Doubling||> 15|