Human Cardiac Fibroblasts (HCF)

Primary Human Cardiac Fibroblasts isolated from the ventricles of the adult heart.

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Primary Human Cardiac Fibroblasts (HCF) are isolated from the ventricles of the adult heart. They play a central role in the maintenance of the extracellular matrix in the normal heart and the synthesis of growth factors and cytokines. Under pathophysiological conditions, cardiac fibroblasts are involved in scar formation after myocardial infarction, cardiac fibrosis, and cardiac hypertrophy. HCF can be used to study such processes in vitro.

Available formats:

  • Cryopreserved: Cryogenic vial containing 500.000 viable cells.
  • Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
  • Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
Recommended Plating Density 3.500 – 7.000 cells per cm2
Passage After Thawing P2
Tested Markers CD90 positive, smooth muscle α-actinin negative, slow muscle myosin negative
Guaranteed Population Doubling > 15
Guaranteed Population Doublings > 15
Figure 1. Human Cardiac Fibroblast Culture in phase contrast.