Have a question about one of our products? Check out our technical library for recently asked questions from other scientists around the world.
Technical Library
Recent Entries in Technical Library
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Is there is a difference in the percentage of differentiated DCs depending on whether we start with cryopreserved or fresh isolated monocytes?
There is no significant difference in the percentage of cells that will differentiate into Dendritic Cells. But when using cryopreserved cells, the initial cell loss will be higher compared to when fresh cells are used. i.e. the final number of differentiated cells that can be expected will be higher with fresh cells as a starting material due to lower cell death rate.
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Can PromoCell release the species the following cytokines were produced in: basic Fibroblast Growth Factor and Epidermial Growth Factor included in the Endothelial Cell Growth Media supplements?
Both cytokines are produced in E.coli.
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What is the differentiation ratio that can be achieved with PromoCell’s subcutaneous HWP?
Most of our subcutaneous preadipocyte lots achieve > 80-90% differentiation when differentiation is induced at P2 (directly after thawing).
We generally recommend using cells for differentiation tests that haven’t undergone more than 4-5 doublings (a maximum of 1 passage after thawing), as the differentiation ratio will decline with the age of the cells.Related Links and Documents
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The HDMEC I purchased from PromoCell seem to contain 2 different cell morphologies. How is this possible? What’s the purity of HDMEC cultures guaranteed by PromoCell?
Our HDMEC are isolated from the dermis of juvenile foreskin or adult skin. The purity is > 95%.
Since the dermis contains blood and lymphatic capillaries, HDMEC cultures comprise blood and lymphatic microvascular endothelial cells that have differing morphologies. Both cell types have a common origin and can be identified by several markers. The ratio of lymphatic and blood derived endothelial cells can vary from lot to lot and is not determined at PromoCell.Related Links and Documents
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What is the basis formula for PromoCell’s Osteoblast Basal Medium (C-27010)?
PromoCell’s Osteoblast Basal Medium is an optimized media formulation developed for human osteoblast culture. The exact composition is proprietary.
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Can I expand the human Mesenchymal Stem Cells prior to differentiating them?
These cells are frozen at the end of 2nd culture. Thawing and seeding results in passage 2 (3rd culture). We recommend that they be used for differentiation experiments not later than passage 5.
The differentiation potential of hMSC in vitro is reduced with ongoing population doublings, meaning the earlier differentiation is induced, the higher the differentiation rates.Related Links and Documents
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What is the lead time when ordering customized media from PromoCell?
The lead time is usually 4-8 weeks.
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Where in the lung are the HPMEC harvested from? Are the cells from arterioles or capillaries?
Our human pulmonary microvascular endothelial cells (C-12281) are sourced from the lung parenchyma with all large vessels being removed beforehand. Therefore, most of the HPMEC originate from capillaries.
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Do the PromoCell HCMEC represent the endocardial cells, i.e. those lining the ventricles? If so, why are they referred to as microvascular endothelial cells?
Our HCMEC (Human Cardiac Microvascular Endothelial Cells) are not endocardial cells. They are isolated from the capillaries in the heart muscle. Therefore, they are in fact microvascular.
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Can I use PBS instead of HepesBSS to wash the cells before trypsinization?
The PromoCell trypsin 0.04% / EDTA 0.03% solution and the PromoCell TNS solution are both based on HepesBSS. Therefore, it is best to also use HepesBSS to wash the cells prior to trypsinization.
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