Products

Macrophages

Human M1 Macrophages (GM-CSF), monocyte-derived
Human M2 Macrophages (M-CSF), monocyte-derived

Primary human macrophages (Mϕ) are hard to isolate from tissue in sufficient amounts with a homogeneous phenotype, do not proliferate in culture, and are difficult to handle in vitro.

PromoCell´s cryopreserved human monocyte-derived macrophages (hMDM) provide a convenient and easy-to-handle solution. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks. Optionally, user-customizable activation of the cells can be performed.

Cryopreserved human macrophages are produced from human monocytes in PromoCell´s well-proven M1/M2- Macrophage Generation Media DXF and are available as non-activated, fully qualified M1- (GM-CSF) or M2- (M-CSF) polarized cells. Each cryo vial contains more than 1.5 million or 5 million viable cells after thawing, respectively.

Figure 1: Flow cytometric anaylsis of PromoCell Cryopreserved Human M1 and M2 Macrophages (click to open large file)

Advantages

  • Assay-ready: Fully functional, adherent M1/M2 macrophages
  • Save time and start your experiments immediately with a defined cell number
  • Wide range of donors available
  • Each lot is tested for: CD68+, CD80+ (M1); CD68+, CD163+ (M2)
  • Generated under xeno-free and defined culture conditions using PromoCell Macrophage Generation Media DXF
  • Easy and convenient protocols for culture and activation available in our Application Notes

 

Figure 2: Overview of macrophage culture with cryopreserved human macrophages, user-customizable activation of the cells is optional. (click to open large file)

 

Step-by-step culture protocol available in our Application Note: Assay-ready adherent cultures of fully functional human primary macrophages using cryopreserved cells.


 

Recommended media & reagents

Related products

References, Questions & Answers

Product Name Size Catalog Number Information Prices
Human M1 Macrophages (GM-CSF), Monocyte-derived, single donor 1,5 Mio cryopreserved cells C-12914 click to select country
Human M1 Macrophages (GM-CSF), Monocyte-derived, single donor 5 Mio cryopreserved cells C-12916 click to select country
Human M2 Macrophages (M-CSF), Monocyte-derived, single donor 1,5 Mio cryopreserved cells C-12915 click to select country
Human M2 Macrophages (M-CSF), Monocyte-derived, single donor 5 Mio cryopreserved cells C-12917 click to select country