Cardiac Myocytes

Primary Human Cardiac Myocytes (HCM) are isolated from the ventricles of the adult heart. They are qualified for in vitro research on cardiac diseases and for pharmacological studies. Unlike freshly isolated rod-shaped myocytes, cultured HCM can be used for long-term experiments like investigating the long-term effects of cytokines, mechanical strain, or cell-cell interactions, as they are prepared according to a special protocol.
Initially, the HCM act more like progenitor cells in that they are not yet fully differentiated. They express the markers of early stage differentiation such as GATA-4 and sarcomeric alpha-actin and have a high capacity for proliferation. When they are grown to confluency and cultivated for an extended period of time, the differentiation process begins. Markers of late differentiation (e.g. sarcomeric alpha-actinin, slow muscle myosin) are increased and the cells begin to form myotube-like structures.

Figure: Flow cytometric analysis of PromoCell HCM after being cultured for 60 days under confluent conditions. 96 % of the cells express sarcomeric a-actinin, a marker of late differentiation.

Recommended media & reagents

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References, Questions & Answers

Product Name Size Catalog Number Information Prices
Human Cardiac Myocytes (HCM) 500,000 cryopreserved cells C-12810 click to select country
Human Cardiac Myocytes (HCM) 500,000 proliferating cells C-12811 click to select country
HCM Pellet 1x106 cells in RNAlater C-14080 click to select country