Macrophage Generation Media DXF

Macrophage Base Medium DXF
M1-Macrophage Generation Medium DXF
M2-Macrophage Generation Medium DXF
Monocyte Attachment Medium

The PromoCell Macrophage Generation Media were developed for easy and efficient generation of the full spectrum of M1- and M2-polarized macrophages directly from freshly isolated peripheral blood monocytes. Detailed step-by-step protocols are available, which allow for fully customized generation of all macrophage activation states.

Key benefits:

  • Easy access to the full range of macrophage activation states
  • Obtain fully functional, untouched macrophages directly from peripheral blood mononuclear cells
  • Generate macrophages from up to 5 buffy coats per medium bottle within one week
  • No time-consuming and costly immunomagnetic purification of monocytes necessary
  • No immunogenic or deleterious effects from animal-derived components

Figure 1. Macrophage Generation Media – Overview (click to enlarge)

Figure 2. How to choose the right medium for your macrophages (click to open as PDF)

Start directly with peripheral blood mononuclear cells

The Monocyte Attachment Medium allows for the efficient adherence selection of monocytes from freshly isolated human mononuclear cells while maintaining optimal cell health. Thus, the time-consuming and costly immunomagnetic purification of monocytes is not necessary.

Ready-to-use: M1-/M2-Macrophage Generation Media DXF

The PromoCell M1-Macrophage Generation Medium DXF contains GM-CSF and allows for the generation of M1-polarized macrophages, whereas the M2-Macrophage Generation Medium DXF contains M-CSF and produces M2-polarized macrophages. Both media are ready-to-use and tested in-house for successful differentiation of M1-/M2-macrophages via flow cytometry.

Fully customizable: Macrophage Base Medium DXF

The Macrophage Base Medium DXF is the user-customizable version of the Macrophage Generation Media DXF and does not include cytokines. It comes without cytokines as a universally applicable MDM culture system featuring a fully user-customizable macrophage differentiation and activation process (see Figure 2).

Defined and xeno-free (DXF)

All PromoCell Macrophage Generation Media DXF are defined and xeno-free media formulations, free of all animal-derived components and substances of human origin with Human Serum Albumin as the only exception.

References, Questions & Answers

Product Name Size Catalog Number Information Prices
Macrophage Base Medium DXF 250 ml C-28057 click to select country
M1-Macrophage Generation Medium DXF (Ready-to-use) 250 ml C-28055 click to select country
M2-Macrophage Generation Medium DXF (Ready-to-use) 250 ml C-28056 click to select country
Monocyte Attachment Medium (Ready-to-use) 250 ml C-28051 click to select country

Problems transfecting your macrophages?

Test our PromoFectin-Macrophage Transfection Reagent!