Primary Cancer Culture System

PromoCell’s Primary Cancer Culture System is a standardized, defined and animal component-free (D-ACF) system for the isolation and culture of human primary tumor cells. It supports the long-term culture of cancer cells while maintaining the clonal diversity of malignant subpopulations. Due to precise stromal control, prolonged culture allows for functional selection of malignant cells. This principle enables access to an enriched population of primary cancer cells.

The Primary Cancer Culture System consists of the Primary Cancer Cell Medium D-ACF, SupplementMix, and NCCD surface treatment reagent.


An innovative solution for culturing primary cancer cells: 

  • Isolate: Reliable technique designed to support a broad range of cancer types
  • Culture: Long-term culture support of patient tumor samples and of patient-derived xenografts (PDX)
  • Purify: Enrichment of primary cancer cells by depletion of non-malignant cells


 Key Advantages:

  • Perform up to 5 isolations with one bottle of medium 
  • Maintains the diversity of original tumor malignant cell subpopulations
  • No stromal overgrowth
  • Defined and animal component-free (D-ACF)

Figure 1: Isolation and culture of primary human cancer cells from tumor specimens using the Primary Cancer Culture System. The optional establishment of cancer cell lines is indicated as well.



Figure 2: Human low-grade small cell lung cancer cells (left; exhibiting a sphere-forming growth pattern) and human squamous carcinoma cells (right: exhibiting an adherent growth pattern) cultured in the Primary Cancer Culture System.


Learn more in our Application Note: Selective In Vitro Culture of Primary Cancer Cells from Human Tumor Samples.




Product Name Size Catalog Number Information Prices
Primary Cancer Culture System 250 ml C-28081 click to select country
NCCD Reagent 2 ml C-43080 click to select country