VEGF-165, mouse, recombinant (E. coli)

Recombinant Mouse Vascular Endothelial Growth Factor 165 (E. coli-derived)

VEGF-165, mouse, recombinant (E. coli)
  10 µg

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VEGF-165, mouse, recombinant (E. coli)

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Recombinant Murine Endothelial Cell Growth Factor (rMu VEGF) is a homodimeric protein secreted by a variety of vascularized tissues. The reported activities of VEGF include stimulation of endothelial cell growth, angiogenesis, and capillary permeability. Murine VEGF is a 39.036 kDa homodimeric, non-glycosylated protein consisting of two 165 amino acid polypeptide chains.

Molecular Mass 39 kDa
Source E. coli
species Mouse
Specific Activity see below
Biological Activity Recombinant murine VEGF165 is fully biologically active when compared to standards. The biological activity of PromoKine's murine VEGF was determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1 to 7 ng/ml.
Formulation Lyophilized from a sterile-filtered, concentrated (1 mg/ml) solution in PBS (pH 7.40) with no additives.
Reconstitution Please Note: Always centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.10 – 1 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Purity Greater than 95% (determined by SDS-PAGE, FPLC and HPLC analysis).
Endotoxin Level < 0.10 ng per µg of rMu VEGF (1EU/µg).
Stability & Storage The lyophilized rMu VEGF, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu VEGF should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.10% endotoxin-free HSA or BSA; e.g. Cat.No. C-69500A). Avoid repeated freeze / thaw cycles.