Transferrin, human, recombinant (Plant)

Recombinant HumanTransferrin (plant-derived)

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Transferrin is the iron-transport protein of vertebrate serum and donates iron to cells through interaction with a specific membrane receptor, CD71. Transferrin appears to be indispensable for most cells growing in tissue culture.
It is referred to frequently as a growth factor because, in analogy to other growth factor-receptor interactions, proliferating cells express high numbers of transferrin receptors, and the binding of transferrin to their receptors is needed for cells to initiate and maintain their DNA synthesis. Apart from its role as an iron transport protein transferrin acts as a cytokine and has functions that may not be related to its iron-carrying capacity.
Recombinant human Transferrin produced in plant is a non-glycosylated, polypeptide chain containing 679 amino acids, having a molecular mass of 76 kDa. It has been purified by proprietary chromatographic techniques.

Molecular Mass 76 kDa
Source Oryza sativa (rice)
Specific Activity see below
Biological Activity One mg of Recombinant Human Transferrin will bind to approximately 2 micrograms of Fe. The average amount of Ferric Iron in our Transferrin is 0.4-0.5ug per mg of protein (protein concentration is measured by absorption at 280 nm).
Formulation The sterile-filtered protein solution (1mg/ml) was lyophilized with no additives
Reconstitution Please Note: Always centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted gently for several minutes in sterile PBS at a concentration of 5-20 mg/ml (avoid bubble formation). This stock solution can then be diluted into other aqueous buffers and stored at –20°C for future use.
Purity Greater than 95% (determined by SDS-PAGE analysis).
Endotoxin Level NA
Stability & Storage The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored dessicated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at –20°C. For long term storage, it is recommended to add a carrier protein (0.1% endotoxin-free HSA or BSA; e.g. Cat.No. C-69500A). Avoid repeated freeze / thaw cycles.