TMRE (2 mM solution in DMSO)
TMRE (Tetramethylrhodamine ethyl ester) and TMRM are preferred dyes to quantitate membrane potentials using the Nernst equation.
Technical Library (1)
What is the principle of the Mitochondrial Apoptosis Staining Kit?
Our Mitochondrial Apoptosis Staining Kit provides a rapid two-color staining procedure performed directly with live cells. The assay quantifies the changes in mitochondrial transmembrane potential, one of the earliest intracellular events upon induction of apoptosis. It uses a cationic fluorescent dye, MitoStain, that fluoresces differently in healthy versus apoptotic cells:
- In non-apoptotic healthy cells, the dye exists as a monomer in the cytosol (green) but also accumulates and aggregates in the mitochondria, emitting a bright red fluorescence
- In apoptotic and necrotic cells, due to disruption of the mitochondrial transmembrane potential, the fluorescent dye only exists as monomer and stains the cytoplasm green.
Analysis of the stained cells can be done by FACS or fluorescence microscopy.
Related Links and Documents
- What is the principle of the Mitochondrial Apoptosis Staining Kit?
Application Notes (0)
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Reference Literature (11)
Improvement of mitochondrial function and lipid utilization by 3,5-dihydroxy-4-methoxybenzyl alcohol, an oyster-derived polyphenol, in oleate-loaded C2C12 myotubes
Ma et al.; Journal of Food and Nutrition Research. 2016;4(8):498-507, Supplementary Material
Tumor microenvironment promotes dicarboxylic acid carrier-mediated transport of succinate to fuel prostate cancer mitochondria
Zhunussova et al.; Am J Cancer Res. 2015 Apr 15;5(5):1665-79
Mitochondria-mediated anticancer effects of non-thermal atmospheric plasma
Zhunussova et al.; PLoS One. 2016 Jun 6;11(6):e0156818
Overexpression of human kynurenine-3-monooxygenase protects against 3-hydroxykynurenine-mediated apoptosis through bidirectional nonlinear feedback
Wilson et al.; Cell Death Dis. 2016 Apr 14;7:e2197
Effects of the novel mitochondrial protein mimitin in insulin-secreting cells
Hanzelka et al.; Biochem J. 2012 Aug 1;445(3):349-59
Identification of cultivation-independent markers of human endothelial cell senescence in vitro
Unterluggauer et al.; Biogerontology. 2007 Aug;8(4):383-97
SK channel activation modulates mitochondrial respiration and attenuates neuronal HT-22 cell damage induced by H2O2
Richter et al.; Neurochem Int. 2015 Feb;81:63-75
Butein-induces apoptosis in human uveal melanoma cells through mitochondrial apoptosis pathway
Cui et al.; Curr Eye Res. 2012 Aug;37(8):730-9
Corosolic acid enhances 5-fluorouracil-induced apoptosis against SNU-620 human gastric carcinoma cells by inhibition of mammalian target of rapamycin
Lee et al.; Mol Med Rep. 2015 Sep;12(3):4782-8
Gallic acid-induced lung cancer cell death is accompanied by ROS increase and glutathione depletion
You et al.; Mol Cell Biochem. 2011 Nov;357(1-2):295-303
- Improvement of mitochondrial function and lipid utilization by 3,5-dihydroxy-4-methoxybenzyl alcohol, an oyster-derived polyphenol, in oleate-loaded C2C12 myotubes
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