The thioredoxin (Trx) system, which is composed of thioredoxin reductase (TrxR), thioredoxin and NADPH, is a key antioxidant system in defense against oxidative stress. Thioredoxin reductase (TrxR) is a ubiquitous enzyme which is also involved in many other cellular processes such as cell growth, p53 activity, cancer, immune response and viral infection.
The PromoKine Thioredoxin-1 (Trx1) Inhibitor Screening Kit provides a fast, convenient and high throughput-adaptable method to screen for Trx1 Inhibitors. In this assay, Trx1 reduces a quenched di-FITC-labeled, oxidized glutathione (Trx1 substrate), to mono-FITC-labeled unquenched, reduced glutathione. In the presence of a a Trx1 inhibitor (control inhibitor is included in the kit), the reaction is impeded or abolished, resulting in lower or no signal.