Sirtuin Activity Assay Kit
This kit provides a rapid, simple, sensitive, and reliable test to measure Sirtuin activity fluorometrically in a variety of samples.
The PromoKine fluorometric Sirtuin Activity Assay Kit provides a rapid, simple, sensitive, and reliable test to measure Sirtuin activity in a variety of samples using a fluorometer or fluorescence multiwell plate reader. In this assay, acetylated p53-AFC substrate is deacetylated by Sirtuins in the presence of NAD to generate the deacetylated p53-AFC substrate which is then cleaved by the developer. The released fluorescent group is detected fluorometrically at Ex/Em = 400/505 nm. The limit of quantification of the assay is 0.06 μU of recombinant human SIRT6.