Our Reduced Glutathione Assay Kit II is based on a specific enzymatic cycling method in the presence of GSH and a fluorophore. The reduction of the fluorophore produces a stable fluorescent product, the fluorescence of which is directly proportional to the amount of GSH in the sample and can be followed kinetically (Ex/Em=535/587 nm). The assay is simple, reproducible and can specifically detect as low as 8 pmol/well of reduced form of Glutathione (GSH) in a 100 µl reaction.
Our kits are the most specific and sensitive quantitative assays in the market because GSSG does not interfere with the assay. The kits include 5-sulfosalicylic acid (SSA) in order to protect samples’ GSH endogenous content (SSA acts as a deproteinizing and antioxidant agent).