The PromoKine Reduced Glutathione Assay Kit I provides a simple and reproducible colorimetric method to specifically detect as low as 50 pmol/well of the reduced form of Glutathione (GSH) in a 100 µl reaction. The assay is based on an enzymatic cycling method in the presence of GSH and a chromophore. The reduction of the chromophore produces a stable product, which can be followed kinetically at 450 nm. Therefore, its absorbance is directly proportional to the amount of GSH in the sample.
Our kits are the most specific and sensitive quantitative assays in the market because GSSG does not interfere with the assay. The kits include 5-sulfosalicylic acid (SSA) in order to protect samples’ GSH endogenous content (SSA acts as a deproteinizing and antioxidant agent).