Reactive Oxygen Species (ROS) Detection Assay Kit
Fluorometric measurement of intracellular ROS levels.
The PromoKine Reactive Oxygen Species (ROS) Detection Kit provides a simple, specific and highly sensitive method for the real-time measurement of ROS levels in living cells or antioxidant activity of test compounds. It utilizes H2DCFDA, a cell-permeable fluorogenic probe, compatible with phenol red, FBS and BSA to detect hydroxyl, peroxyl, or other reactive oxygen species in live cells. Upon the cell entry, H2DCFDA is modified by cellular esterases to form a non-fluorescent H2DCF which is oxidized by intracellular ROS to yield a highly fluorescent product that can be detected by using FACS, microplate reader, or fluorescence microscope (Ex/Em 495/529 nm). The fluorescence intensity is proportional to the ROS levels.