Proteasome Activity Assay Kit
Simple, rapid and accurate fluorometric assay to specifically measure proteasome activity present in biological samples such as cell lysates/extracts.
PromoCell’s Proteasome Activity Assay takes advantage of the chymotrypsin-like activity, utilizing an AMC-tagged peptide substrate which releases free, highly fluorescent AMC in the presence of proteolytic activity. The kit also includes a positive control (Jurkat cell lysate with significant proteasome activity) and a specific proteasome inhibitor (MG-132) which suppresses all proteolytic activity due to proteasomes. This permits differentiation of proteasome activity from other protease activity which may be present in samples.