PromoFluor LSS Maleimides

PromoFluor Maleimides for labeling thiol groups of proteins exhibiting an exceptional large Stokes shift

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In addition to the standard dyes, specifically designed PromoFluor LSS dyes offer an exceptional large Stokes shift, which makes them ideally suited for multicolor labelling and flow cytometry applications.
PromoFluor Maleimides are intended for covalently labeling biomolecules containing a thiol group (e.g. proteins). A neutral thiol-reactive label has little influence on the isoelectric point of a protein. In effect, PromoFluor Maleimides have an advantage over amino group labels because they do not interfere with techniques like differential 2D gel electrophoresis. Since most proteins contain only a few thiol functional groups, thiol-reactive dyes can be used to selectively modify a protein or peptide containing a thiol group at a specific site. Moreover, these dyes are well suited for labelling oligonucleotides. Maleimides couple to thiol groups under very mild conditions at a pH around 7.

PromoFluor LSS Dyes (Large-Stokes-Shift Dyes)
| Label | Absorption Maximum [nm] | Emission Maximum [nm] | Laser Excitation Source |
|————|————————-|———————–|————————-|
| PF-350-LSS | 349 | 610 | Argon laser, UV light |
| PF-370-LSS | 368 | 473 | Argon laser, UV light |
| PF-375-LSS | 376 | 543 | Argon laser, UV light |
| PF-380-LSS | 381 | 510 | Argon laser, UV light |
| PF-395-LSS | 396 | 572 | Argon laser, UV light |
| PF-488-LSS | 485 | 560 | Argon 488 laser |
| PF-500-LSS | 500 | 630 | Argon 488 laser |
| PF-510-LSS | 509 | 590 | Argon 514 laser |
| PF-514-LSS | 515 | 650 | Argon 514 laser |
| PF-520-LSS | 520 | 664 | Argon 514 laser |