Cell-specific transfection reagent (hepatocytes)

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PromoFectin-Hepatocyte has been designed for transfection of cells expressing galactose-specific membrane lectins and has been very successfully used to transfect primary hepatocytes as well as hepatocyte cell lines (e.g. BNL CL.2) and human hepatocarcinoma cells (e.g. HepG2) with up to 50% transfection efficiency and excellent cell viability. This PromoFectin derivative specifically targets cell surface receptors, leading to internalization of the complexed nucleic acids.

Figure 1. Primary human hepatocytes transfected with a GFP reporter plasmid using PromoFectin-Hepatocyte.
Figure 2. HepG2 cells were transfected with a GFP reporter vector using PromoFectin Hepatocyte (right) and E43 competing reagents (Lipofectamine 2000, left and Lipofectamine LTX, middle). PromoFectin-Hepatocyte showed the best transfection rate (~30%).