M2-Macrophage Generation Medium DXF

Defined and xeno-free medium for generation of M2 macrophages from fresh monocytes.

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M2-Macrophage Generation Medium DXF
  250 ml
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The PromoCell Macrophage Generation Media have been developed for the efficient generation of monocyte-derived macrophages (MDM) from freshly isolated peripheral blood monocytes or directly from PBMC as a starting material. The M2-Macrophage Generation Medium DXF contains M-CSF and allows for the generation of M2-polarized macrophages. PromoCells M2-Macrophage Generation Medium DXF is a ready-to-use medium including cytokines for the directed differentiation of M2-like polarized MDM.

The Macrophage Media DXF are defined and xeno-free formulations for use with freshly isolated cells. Due to the utilization of exclusively synthetic, recombinant or plant-sourced materials, this medium is free of all animal-derived components and substances of human origin with human serum albumin as the only exception.


  • Basal Medium M2 (250ml)
  • SupplementMix M2 (for 250ml)
  • CytokineMix M2: M-CSF (for 250ml)
Macrophage Generation
Figure 1. In vitro macrophage generation with the PromoCell macrophage media system. Overview of the wide spectrum of macrophage polarization and activation possibilities.