Luciferase Reporter Assay Kit III (Firefly & Renilla Single-tube Assay)

Ready-to-use kit for rapid, convenient and quantitative detection of Firefly and Renilla luciferase activity in eukaryotic cells.

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Firefly and Renilla luciferases are widely used as reporter genes for studying gene regulation and function, and for pharmaceutical screening. The PromoKine ready-to-use Luciferase Reporter Assay Kits provide complete systems for rapid, convenient, and quantitative detection of Firefly and Renilla luciferase activity in cultured eukaryotic cells, e.g., cells transfected with a plasmid carrying the Firefly and Renilla luciferase reporter genes. They are optimized to provide excellent sensitivity as well as consistent light output when working with multiple samples.

Renilla luciferase is often used in conjunction with Firefly luciferase as a normalizing transfection control or for multiplex transcriptional reporter assays. Here, our improved Luciferase Reporter Assay Kit III (Firefly & Renilla Single-tube Assay) provides optimal convenience and highest sensitivity.
Both, Firefly luciferase and Renilla luciferase show maximum light output only when substrates and co-factors are present in large quantities. When assayed under these conditions, light emitted from the reaction is directly proportional to the number of luciferase enzyme molecules. The light emission from the reaction can be recorded using a luminometer.