Luciferase Reporter Assay Kit I (Firefly)

Ready-to-use kit for rapid, convenient and quantitative detection of Firefly luciferase activity in eukaryotic cells.

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Firefly luciferases are widely used as reporter genes for studying gene regulation and function, and for pharmaceutical screening. The PromoKine ready-to-use Luciferase Reporter Assay Kit I provides a complete systems for rapid, convenient, and quantitative detection of Firefly luciferase activity in cultured eukaryotic cells, e.g., cells transfected with a plasmid carrying the Firefly luciferase reporter genes. They are optimized to provide excellent sensitivity as well as consistent light output when working with multiple samples.

Firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any known bioluminescence reaction. Consequently, this sensitive reporter is ideally suited for studying gene regulation and function.
Firefly luciferase shows maximum light output only when substrates and co-factors are present in large quantities. When assayed under these conditions, light emitted from the reaction is directly proportional to the number of luciferase enzyme molecules. The light emission from the reaction can be recorded using a luminometer.