LDH Cytotoxicity Kit II (colorimetric)

Colorimetric cytotoxicity detection

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LDH Cytotoxicity Kit II (colorimetric)
PK-CA577-K313
  500 assays
$369,00

Based on the activity of lactate dehydrogenase (LDH), our colorimetric LDH Cytotoxicity Kit provides a fast, sensitive, and precise alternative to the radioactive [3H]-thymidine and [51Cr] release assays.
LDH is a stable cytoplasmic enzyme present in all cells and is rapidly released into the surrounding medium when cells are damaged. In this assay, LDH activity is determined by a coupled enzymatic reaction. LDH catalyses the conversion of lactate to pyruvate, triggering an enzymatic reaction that produces a water-soluble red formazan dye. The increase in the amount of produced in culture medium correlates directly to the increase in the number of lysed cells and can be analyzed spectrophotometrically. The kits can be used for many different in vitro cell systems to quantify cytotoxicity and cytolysis in fast and convenient assay

Figure 1. Assay mechanisms of the PromoKine LDH Cytotoxicity Kit II.
Figure 1. Assay mechanisms of the PromoKine LDH Cytotoxicity Kit II.