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Primary Human White Preadipocytes (HWP) are isolated from adult subcutaneous or visceral adipose tissue from different locations (lot specific source information is available on request).
Adipose tissue is crucial in energy storage and metabolic homeostasis. An increase in adipose tissue results either from an enlargement of mature adipocytes or from the differentiation of adipocyte precursor cells (preadipocytes) into new mature adipocytes. These preadipocytes are already present in adipose tissues. They can proliferate throughout adult life and replace the cells that have differentiated into mature adipocytes.
Since this differentiation process is controlled by a variety of growth factors and hormones, preadipocytes are suitable for the investigation of the physiological mechanisms controlling proliferation, differentiation, and function of adipose tissue.
The PromoCell adipocyte cell culture system provides preadipocytes with optimized growth media and serum-free differentiation media.
- Cryopreserved: Cryogenic vial containing 500.000 visceral and 1.000.000 subcutaneous viable cells.
- Proliferating: >500.000 visceral and 1.000.000 subcutaneous viable cells shipped in growth medium (T25 flask).
- Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
|Recommended plating density||5,000 cells per cm2|
|Passage After Thawing||P2|
|Tested Markers||Differentiation into adipocytes tested|
|Guaranteed Population Doubling||> 10|