Human Uterine Microvascular Endothelial Cells (HUtMEC)
Primary Human Uterine Microvascular Endothelial Cells isolated from the myometrium from a single donor. Donors not pre-treated with hormones.
Primary Human Uterine Microvascular Endothelial Cells (HUtMEC) are isolated from the myometrium (middle layer of the uterine wall) from a single donor who is not pre-treated with hormones. The cells are routinely analyzed by immunofluorescent staining: they stain positive for CD31 and von Willebrand factor and negative for smooth muscle alpha-actin.
During pregnancy HUtMEC propagate intensely and change their phenotype and expression pattern in response to sex steroids. This includes up-regulation of HUtMEC nitric oxide synthase activity, modulation of adhesion molecule expression, and an increase in endothelial cell survival and angiogenic activity. HUtMEC are involved in medical conditions like uterine fibroma or adeno-mysis.
|Recommended Plating Density||10.000 – 20.000 cells per cm2|
|Passage After Thawing||P2|
|Tested Markers||von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positive|
|Guaranteed Population Doublings||> 15|
Technical Library (1)
From what tissue do PromoCell isolate their HUtMEC (C-12295)?
Our HUtMEC are isolated from the middle layer of the uterine wall (myometrium). The tissue donors were not pre-treated with hormones.
Related Links and Documents
- From what tissue do PromoCell isolate their HUtMEC (C-12295)?
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Reference Literature (3)
Antihypertensive methyldopa, labetalol, hydralazine, and clonidine reversed tumour necrosis factor-alpha inhibited endothelial nitric oxide synthase expression in endothelial-trophoblast cellular networks
Xu et al.; Clin Exp Pharmacol Physiol. 2017 Mar;44(3):421-427
Transendothelial migration of human umbilical mesenchymal stem cells across uterine endothelial monolayers: junctional dynamics and putative mechanisms
Ebrahim and Leach; Placenta. 2016 Dec;48:87-98
Nitric oxide (NO) reversed TNF-alpha inhibition of trophoblast interaction with endothelial cellular networks
Xu et al.; Placenta. 2014 Jun;35(6):417-21
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