Human Umbilical Artery Smooth Muscle Cells (HUASMC)
Primary Human Umbilical Artery Smooth Muscle Cells isolated from the arteries of the umbilical cord.
Primary Human Umbilical Artery Smooth Muscle Cells (HUASMC) are isolated from the arteries of the umbilical cord and stain positive for smooth muscle α-actin. Physiological and biochemical processes of vascular diseases can be analyzed with HUASMC in culture.
NEW: Our HUASMC are now also available from HLA-typed donors.
- Cryopreserved: Cryogenic vial containing 500.000 viable cells.
- Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
- Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
|Recommended plating density||7,500 – 10,000 cells per cm2|
|Passage After Thawing||P2|
|Tested Markers||Smooth muscle α-Actin positive|
|Guaranteed Population Doubling||> 15|
Technical Library (1)
How long does it take to detach primary cells with accutase (C-41310)?
Most primary cells detach within 5-10 min at 37°C. Inactivation isn’t required but we recommend to centrifuge the cell suspension to remove accutase and EDTA before replating the cells.
Related Links and Documents
- How long does it take to detach primary cells with accutase (C-41310)?
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Reference Literature (10)
Proteomic analysis of vascular smooth muscle cells in physiological condition and in pulmonary arterial hypertension: toward contractile versus synthetic phenotypes
Regent et al.; Proteomics. 2016 Oct;16(20):2637-2649
PPARgamma activation inhibits cerebral arteriogenesis in the hypoperfused rat brain
Duelsner et al.; Acta Physiol (Oxf). 2014 Feb;210(2):354-68
The “Artificial Artery” as in vitro perfusion model
Janke et al.; PLoS ONE. 2013;8(3):e57227
5-methoxyleoligin, a lignan from Edelweiss, stimulates CYP26B1-dependent angiogenesis in vitro and induces arteriogenesis in infarcted rat hearts in vivo
Messner et al.; PLoS One. 2013;8(3):e58342
Blood vessel maturation in a 3-dimensional spheroidal coculture model: direct contact with smooth muscle cells regulates endothelial cell quiescence and abrogates VEGF responsiveness
Korff et al.; FASEB J. 2001 Feb;15(2):447-57
A switch toward angiostatic gene expression impairs the angiogenic properties of endothelial progenitor cells in low birth weight pre-term infants
Ligi et al.; Blood. 2011 Aug 11;118(6):1699-709
Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells
Füller et al.; J Cell Sci. 2003 Jun 15;116(Pt 12):2461-70
Intrinsic versus microenvironmental regulation of lymphatic endothelial cell phenotype and function
Veikkola et al.; FASEB J. 2003 Nov;17(14):2006-13
The Tie-2 ligand Angiopoietin-2 is stored in and rapidly released upon stimulation from endothelial cell Weibel-Palade bodies
Fiedler et al.; Blood. 2004 Jun 1;103(11):4150-6
The Tie-2 ligand Angiopoietin-2 destabilizes quiescent endothelium through an internal autocrine loop mechanism
Scharpfenecker et al. J Cell Sci. 2005 Feb 15;118(Pt 4):771-80
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