Human Tracheal Smooth Muscle Cells (HTSMC)

Primary Human Tracheal Smooth Muscle Cells isolated from the trachea from single donors.

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Primary Human Tracheal Smooth Muscle Cells (HTSMC) are isolated from the trachea from single donors and stain positive for smooth muscle α-actin. They connect the endings of the tracheal cartilage by a contractile bridge called the paries membranaceus and consequently regulate the airway lumen. Under pathological conditions like asthma, the amount of smooth muscle cells in the trachea walls is increased leading to smooth muscle hypertrophy or hyperplasia. Hence, HTSMC are suitable for in vitro studies of asthma and other pulmonary diseases.

Available formats:

  • Cryopreserved: Cryogenic vial containing 500.000 viable cells.
  • Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
  • Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
Recommended Plating Density 7.500 – 10.000 cells per cm2
Passage After Thawing P2
Tested Markers Smooth muscle α-Actin positive
Guaranteed Population Doubling > 15
Guaranteed Population Doublings > 15
Figure 1. Human Tracheal Smooth Musce Cell Culture in phase contrast.
Figure 2. HTSMC culture stained for smooth muscle alpha-actin. Nuclei counterstained with DAPI.