Human Saphenous Vein Endothelial Cells (HSaVEC)
Primary Human Saphenous Vein Endothelial Cells isolated from saphenous veins from single donors.
Primary Human Saphenous Vein Endothelial Cells (HSaVEC) are isolated from human saphenous veins from single donors. The saphenous vein is often used for autotransplantation in coronary artery bypass operations, when arterial grafts are not available or many grafts are required.
NEW: Our HSaVEC are now also available from HLA-typed donors.
- Cryopreserved: Cryogenic vial containing 500.000 viable cells.
- Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
- Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
|Recommended plating density||5,000 – 10,000 cells per cm2|
|Passage After Thawing||P2|
|Tested Markers||von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positive|
|Guaranteed Population Doubling||> 15|
Technical Library (2)
From which part of the leg does PromoCell isolate the HSaVEC?
The Vena saphena section that we use for HSaVEC isolation originates from the thigh.
Which medium do PromoCell recommend to use on HUVEC – Endothelial Cell Growth Medium or Endothelial Cell Growth Medium 2?
The standard medium for isolation and propagation of our HUVEC, HUAEC, HPAEC, and HSaVEC is Endothelial Cell Growth Medium (C-22010). It contains ECGS, an extract from bovine hypothalamus which has mitogenic effects on endothelial cell proliferation. Scientists who prefer a more defined Growth Medium can use Endothelial Cell Growth Medium 2 (C-22011). In this medium, ECGS is replaced by VEGF, IGF, and additional bFGF and EGF to stimulate endothelial cell growth.
Related Links and Documents
- From which part of the leg does PromoCell isolate the HSaVEC?
Application Notes (0)
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Reference Literature (11)
Specific gene transfer to neurons, endothelial cells and hematopoietic progenitors with lentiviral vectors
Anliker et al.; Nat Methods. 2010 Nov;7(11):929-35
Effect of HMGCoA reductase inhibitors on cytochrome P450 expression in endothelial cell line
Bertrand-Thiebault et al.; J Cardiovasc Pharmacol. 2007 May;49(5):306-15
Modulation of human vascular endothelial cell behaviors by nanotopographic cues
Liliensiek et al.; Biomaterials. 2010 Jul;31(20):5418-26
Endothelial cells obtained from patients affected by chronic venous disease exhibit a pro-inflammatory phenotype
Tisato et al.; PLoS One. 2012;7(6):e39543
Direct effects of pure nicotine, cigarette smoke extract, Swedish-type smokeless tobacco (Snus) extract and ethanol on human normal endothelial cells and fibroblasts
Laytragoon-Lewin et al.; Anticancer Res. 2011 May;31(5):1527-34
Causal relationship between hyperfibrinogenemia, thrombosis, and resistance to thrombolysis in mice
Machlus et al.; Blood. 2011 May 5;117(18):4953-63
The role of substratum compliance of hydrogels on vascular endothelial cell behavior
Wood et al.; Biomaterials. 2011 Aug;32(22):5056-64
The Tie-2 ligand Angiopoietin-2 is stored in and rapidly released upon stimulation from endothelial cell Weibel-Palade bodies
Fiedler et al.; Blood. 2004 Jun 1;103(11):4150-6
Intrinsic versus microenvironmental regulation of lymphatic endothelial cell phenotype and function
Veikkola et al.; FASEB J. 2003 Nov;17(14):2006-13
Vascular bed origin dictates flow pattern regulation of endothelial adhesion molecule expression
Methe et al.; Am J Physiol Heart Circ Physiol. 2007 May;292(5):H2167-75
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Endothelial Cell TypesWant to know more about our portfolio of endothelial cells? Take a look at our poster for an overview of our large-vessel and microvascular endothelial cells, and where they are located in the body.
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