Human Saphenous Vein Endothelial Cells (HSaVEC)
Primary Human Saphenous Vein Endothelial Cells isolated from saphenous veins from single donors.
Primary Human Saphenous Vein Endothelial Cells (HSaVEC) are isolated from human saphenous veins from single donors. The saphenous vein is often used for autotransplantation in coronary artery bypass operations, when arterial grafts are not available or many grafts are required.
- Cryopreserved: Cryogenic vial containing 500.000 viable cells.
- Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
- Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
|Recommended Plating Density||5000 – 10000 cells per cm2|
|Passage After Thawing||P2|
|Tested Markers||von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positive|
|Guaranteed Population Doubling||> 15|
|Guaranteed Population Doublings||> 15|
Technical Library (2)
From which part of the leg does PromoCell isolate the HSaVEC?
The Vena saphena section that we use for HSaVEC isolation originates from the thigh.
Which medium do PromoCell recommend to use on HUVEC – Endothelial Cell Growth Medium or Endothelial Cell Growth Medium 2?
The standard medium for isolation and propagation of our HUVEC, HUAEC, HPAEC, and HSaVEC is Endothelial Cell Growth Medium (C-22010). It contains ECGS, an extract from bovine hypothalamus which has mitogenic effects on endothelial cell proliferation. Scientists who prefer a more defined Growth Medium can use Endothelial Cell Growth Medium 2 (C-22011). In this medium, ECGS is replaced by VEGF, IGF, and additional bFGF and EGF to stimulate endothelial cell growth.
Related Links and Documents
- From which part of the leg does PromoCell isolate the HSaVEC?
Application Notes (0)
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