Human Pulmonary Microvascular Endothelial Cells (HPMEC)

Primary Human Pulmonary Microvascular Endothelial Cells isolated from the lung from a single donor.

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Selected product Human Pulmonary Microvascular Endothelial Cells (HPMEC)

cryopreserved 500,000 cells

proliferating 500,000 cells

cell pellet in RNAlater 1,000,000 cells

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Primary Human Pulmonary Microvascular Endothelial Cells (HPMEC) are isolated from the lung from a single donor. Since lung tissue contains blood and lymphatic capillaries, HPMEC comprise Blood and Lymphatic Microvascular Endothelial Cells. The cells are routinely analyzed by immunofluorescent staining: they stain positive for CD31 and von Willebrand factor and negative for smooth muscle alpha-actin.

The lung has a vast endothelial surface area, which is essential for the exchange of gases. HPMEC are most appropriate for studying human lung diseases.

NEW: Our HPMEC are now also available from HLA-typed donors.

Available formats:

  • Cryopreserved: Cryogenic vial containing 500.000 viable cells.
  • Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
  • Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
Recommended plating density 10,000 – 20,000 cells per cm2
Passage After Thawing P2
Tested Markers von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positive
Guaranteed Population Doubling > 15

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