Primary Human Pulmonary Microvascular Endothelial Cells (HPMEC) are isolated from the lung from a single donor. Since lung tissue contains blood and lymphatic capillaries, HPMEC comprise Blood and Lymphatic Microvascular Endothelial Cells. The cells are routinely analyzed by immunofluorescent staining: they stain positive for CD31 and von Willebrand factor and negative for smooth muscle alpha-actin.
The lung has a vast endothelial surface area, which is essential for the exchange of gases. HPMEC are most appropriate for studying human lung diseases.
NEW: Our HPMEC are now also available from HLA-typed donors.
- Cryopreserved: Cryogenic vial containing 500.000 viable cells.
- Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
- Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
|Recommended plating density||10,000 – 20,000 cells per cm2|
|Passage After Thawing||P2|
|Tested Markers||von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positive|
|Guaranteed Population Doubling||> 15|