Human Pulmonary Fibroblasts (HPF)
Primary Human Pulmonary Fibroblasts isolated from human lung tissue.
Primary Human Pulmonary Fibroblasts (HPF) are isolated from human lung tissue. They are often used for studying lung tissue repair, tissue remodeling after injury, or the response after pulmonary inflammation. Fibroblast disorders like pulmonary fibrosis or hypoxia-induced pulmonary hypertension can also be investigated with cultured HPF.
- Cryopreserved: Cryogenic vial containing 500.000 viable cells.
- Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
- Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
|Recommended Plating Density||3.500 – 7.000 cells per cm2|
|Passage After Thawing||P2|
|Tested Markers||CD90 positive|
|Guaranteed Population Doubling||> 15|
|Guaranteed Population Doublings||> 15|
Technical Library (1)
From what part of the lungs does PromoCell isolate the human pulmonary fibroblasts (HPF)?
Our HPF (C-12360) are isolated from peripheral lung tissue.
Related Links and Documents
- From what part of the lungs does PromoCell isolate the human pulmonary fibroblasts (HPF)?
Application Notes (0)
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Reference Literature (20)
MicroRNA-29c regulates apoptosis sensitivity via modulation of the cell-surface death receptor, Fas, in lung fibroblasts
Matsushima and Ishiyama; Am J Physiol Lung Cell Mol Physiol. 2016 Dec 1;311(6):L1050-L1061
Pyrogallol induces the death of human pulmonary fibroblast cells through ROS increase and GSH depletion
Park; Int J Oncol. 2016 Aug;49(2):785-92
Role of CD248 as a potential severity marker in idiopathic pulmonary fibrosis
Bartis et al.; BMC Pulm Med. 2016 Apr 14;16(1):51
Antimycin A induces death of the human pulmonary fibroblast cells via ROS increase and GSH depletion
Park and You; Int J Oncol. 2016 Feb;48(2):813-20
Down-regulation of thioredoxin1 is involved in death of Calu-6 lung cancer cells treated with suberoyl bishydroxamic acid
You et al.; J Cell Biochem. 2016 May;117(5):1250-61
Involvement of Kruppel-like factor 9 in bleomycin-induced pulmonary toxicity
Gu et al.; Mol Med Rep. 2015 Oct;12(4):5262-6
Effects of silver and gold nanoparticles of different sizes in human pulmonary fibroblasts
Avalos et al.; Toxicol Mech Methods. 2015 Mar 23:1-9
Zebularine inhibits the growth of A549 lung cancer cells via cell cycle arrest and apoptosis
You and Park; Mol Carcinog. 2014 Nov;53(11):847-57
CCL2 mediates anti-fibrotic effects in human fibroblasts independently of CCR2
Kalderen et al.; Int Immunopharmacol. 2014 May;20(1):66-73
Demonstration of a melanoma-specific CD44 alternative splicing pattern that remains qualitatively stable, but shows quantitative changes during tumour progression
Raso-Barnett et al.; PLoS One. 2013;8(1):e53883
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