Human Osteoblasts (HOB)

Primary Human Osteoblasts isolated from femoral trabecular bone tissue from the knee or hip joint region.

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Primary Human Osteoblasts (HOB) are isolated from femoral trabecular bone tissue from the knee or hip joint region (lot specific source information is available on request). They stain positive for alkaline phosphatase and can be mineralized using PromoCell Mineralization Medium.

Osteoblasts are of mesenchymal origin and are mainly responsible for bone formation and remodeling. In vivo, they produce a collagen type I rich extracellular matrix called the osteoid.

During their development, osteoblasts undergo a maturation process called osteogenesis. When they become encased in the bone matrix, they stop proliferating and become osteocytes.

Available formats:

  • Cryopreserved: Cryogenic vial containing 500.000 viable cells.
  • Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
  • Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
Recommended Plating Density 10.000 – 20.000 cells per cm2
Passage After Thawing P2
Tested Markers Alkaline phosphates positive, Mineralization tested
Guaranteed Population Doubling > 10
Guaranteed Population Doublings > 10
Figure 1. Human Osteoblast Cell Culture in phase contrast
Figure 2. Alkaline phosphatase activity in HOB stained with BCIP/NBT.
Figure 3. Mineralized HOB. Calcium deposit stained with Alizarin Red S.