Human Mononuclear Cells (hMNC)

Primary Human Mononuclear Cells isolated from umbilical cord blood and peripheral blood.

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PromoCell Mononuclear Cells (MNC) represent the lymphocyte and monocyte fraction isolated from umbilical cord blood or peripheral blood. They are prepared by optimized low-density gradient centrifugation, which effectively reduces the granulocyte content. Red blood cells are gently but thoroughly depleted by proprietary techniques instead of osmotic lysis. This results in ultra-pure mononuclear cells with superior viability and unchanged biological function. In addition, the cells do not clump after thawing, allowing for easy handling and reproducible results.

The MNC can be used directly for experiments or as a starting material for the isolation of several cell types, e.g., hematopoietic and endothelial progenitors, monocytes, and lymphocyte subpopulations.

Available formats:

  • Cryopreserved: Cryogenic vial containing 500.000 viable cells.
  • Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
  • Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
Recommended Plating Density 1.000.000 cells per ml
Tested Markers CD31 negative, CD34 negative, CD105 positive, CD146 positive
Figure 1. Flow cytometric analysis of Mononuclear Cells from Cord Blood (hMNC-CB). Granulocytes content 1%.
Figure 2. Flow cytometric determination of CD34 cells within the PromoCell Mononuclear Cells from Cord Blood (hMNC-CB)