Human Chondrocytes (HCH)

Primary Human Chondrocytes isolated from normal human articular cartilage from the knee and hip joints.

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Primary Human Chondrocytes (HCH) are isolated from normal human articular cartilage from the knee and hip joints (lot specific source information is available on request). PromoCell HCH are routinely tested for their capacity to differentiate in 3D spheroids.

The articular cartilage covers the joints between bones and contains no blood vessels, lymphatic vessels, or nerve fibers. It is composed of only one specialized cell type, the chondrocytes, which produce and maintain the extracellular matrix of cartilage, i.e. collagen (mostly type II) and proteoglycans (primarily aggrecan).

Available formats:

  • Cryopreserved: Cryogenic vial containing 500.000 viable cells.
  • Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
  • Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
Recommended Plating Density 10.000 – 20.000 cells per cm2
Passage After Thawing P2
Tested Markers Differentiation into 3D spheroids tested
Guaranteed Population Doubling > 10
Guaranteed Population Doublings > 10
Figure 1. Human Chondrocyte Cell Culture in phase contrast
Figure 2. Spheroids of differentiated HCH cultures in Chondrocyte Growth Medium and stained with Alcian Blue.