Human Cardiac Myocytes (HCM)

Primary Human Cardiac Myocytes isolated from the ventricles of the adult heart.

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Primary Human Cardiac Myocytes (HCM) are isolated from the ventricles of the adult heart. They are qualified for in vitro research on cardiac diseases and for pharmacological studies. Unlike freshly isolated rod-shaped myocytes, cultured HCM can be used for long-term experiments like investigating the long-term effects of cytokines, mechanical strain, or cell-cell interactions, as they are prepared according to a special protocol.

Initially, the HCM act more like progenitor cells in that they are not yet fully differentiated. They express the markers of early stage differentiation such as GATA-4 and sarcomeric alpha-actin and have a high capacity for proliferation. When they are grown to confluency and cultivated for an extended period of time, the differentiation process begins. Markers of late differentiation (e.g. sarcomeric alpha-actinin, slow muscle myosin) are increased and the cells begin to form myotube-like structures.

Available formats:

  • Cryopreserved: Cryogenic vial containing 500.000 viable cells.
  • Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
  • Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
Recommended Plating Density 10.000 – 15.000 cells per cm2
Passage After Thawing P2
Tested Markers Sarcomeric alpha-Actinin positive, Slow muscle myosin positive
Guaranteed Population Doubling > 15
Guaranteed Population Doublings > 15
Figure 1. Human Cardiac Muscle Cell Culture in phase contrast
Figure 2. Flow cytometric analysis of PromoCell HCM after being cultured for 60 days under confluent conditions. 96% of the cells express sarcomeric alpha-actinin, a marker of late differentiation.