Human Cardiac Microvascular Endothelial Cells (HCMEC)
Primary Human Cardiac Microvascular Endothelial Cells isolated from heart ventricles from a single donor.
Primary Human Cardiac Microvascular Endothelial Cells (HCMEC) are isolated from heart ventricles from a single donor. The cells are routinely analyzed by immunofluorescent staining: they stain positive for CD31 and Willebrand factor and negative for smooth muscle alpha-actin.
HCMEC intensively interact with cardiomyocytes and therefore have a characteristic phenotype different from other microvascular endothelial cells. HCMEC play an important role in the physiological regulation of coronary blood flow and capillary exchange. Medical conditions such as hypertrophy, ischemia, hypertension, and diabetes mellitus are associated with endothelial dysfunction.
- Cryopreserved: Cryogenic vial containing 500.000 viable cells.
- Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
- Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
|Recommended Plating Density||10.000 – 20.000 cells per cm2|
|Passage After Thawing||P2|
|Tested Markers||von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positive|
|Guaranteed Population Doubling||> 10|
|Guaranteed Population Doublings||> 10|
Technical Library (2)
Do the PromoCell HCMEC represent the endocardial cells, i.e. those lining the ventricles? If so, why are they referred to as microvascular endothelial cells?
Our HCMEC (Human Cardiac Microvascular Endothelial Cells) are not endocardial cells. They are isolated from the capillaries in the heart muscle. Therefore, they are in fact microvascular.
Related Links and Documents
Which medium does PromoCell recommend for endothelial cell transfection ?
There are several factors that can influence successful transfections, e.g. viability and density of the cells, choice of the transfection reagent, quality and type of the transfected molecules (plasmids, siRNA, oligonucleotides), as well as the culture medium and supplements used.
When using PromoCell Endothelial Cell Growth Media for cell transfection, please follow the instructions below:
Heparin, which is included in our Endothelial Cell Growth Medium (C-22010/C-22110), Medium 2 (C-22011/C-22111) and Medium MV (C-22020/C-22120) may reduce the transfection efficiency. We therefore recommend to use our heparin-free Endothelial Cell Growth Medium MV2 (C-22022/C-22121). Alternatively, you may use Endothelial Cell Growth Medium Kit (C-22110), Medium 2 Kit (C-22111), or Medium MV Kit (C-22120) without adding the heparin supplement to the Basal Medium.
Please note: Before and after transfection, the cells should be cultured in complete Growth Medium including heparin to ensure optimal growth.
Related Links and Documents
- Do the PromoCell HCMEC represent the endocardial cells, i.e. those lining the ventricles? If so, why are they referred to as microvascular endothelial cells?
Application Notes (0)
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Reference Literature (15)
Human pluripotent stem cell-derived epicardial progenitors can differentiate to endocardial-like endothelial cells
Bao et al.; Bioengineering & Translational Medicine. 2017;2:191-201
PAI1: a novel PP1-interacting protein that mediates human plasma’s anti-apoptotic effect in endothelial cells
Yao et al.; J Cell Mol Med. 2017 Sep;21(9):2068-2076
Cardiotoxic drugs Herceptin and doxorubicin inhibit cardiac microvascular endothelial cell barrier formation resulting in increased drug permeability
Wilkinson et al.; Biol Open. 2016 Oct 15;5(10):1362-1370
Exosomes increase the therapeutic index of doxorubicin in breast and ovarian cancer mouse models
Hadla et al.; Nanomedicine (Lond). 2016 Sep;11(18):2431-41
The arachidonate 15-lipoxygenase enzyme product 15-HETE is present in heart tissue from patients with ischemic heart disease and enhances clot formation
Lundqvist et al.; PLoS One. 2016 Aug 23;11(8):e0161629
Cardiac non-myocyte cells show enhanced pharmacological function suggestive of contractile maturity in stem cell derived cardiomyocyte microtissues
Ravenscroft et al.; Toxicol Sci. 2016 Jul;152(1):99-112
Hypoxia-induced endothelial-mesenchymal transition is associated with RASAL1 promoter hypermethylation in human coronary endothelial cells
Xu et al.; FEBS Lett. 2016 Apr;590(8):1222-33
A specifically designed nanoconstruct associates, internalizes, traffics in cardiovascular cells, and accumulates in failing myocardium: a new strategy for heart failure diagnostics and therapeutics
Ruiz-Esparza et al.; Eur J Heart Fail. 2016 Feb;18(2):169-78
Endothelial Gata5 transcription factor regulates blood pressure
Messaoudi et al.; Nat Commun. 2015 Nov 30;6:8835
Role of mitochondrial dysfunction in hyperglycaemia-induced coronary microvascular dysfunction: Protective role of resveratrol
Joshi et al.; Diab Vasc Dis Res. 2015 May;12(3):208-16
- Human pluripotent stem cell-derived epicardial progenitors can differentiate to endocardial-like endothelial cells
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