Human Aortic Smooth Muscle Cells (HAoSMC)
Primary Human Aortic Smooth Muscle Cells isolated from the human aorta.
Primary Human Aortic Smooth Muscle Cells (HAoSMC) are isolated from plaque-free regions of the human aorta and stain positive for smooth muscle α-actin. The pulsatile pressure produced by the heart causes a cyclic distention of the aorta. The smooth muscle cells in the aortic wall subsequently contract it again. Changes in the arterial wall, associated with vascular diseases such as atherosclerosis and hypertension, strongly influence this process.
HAoSMC are suitable for studying the role of smooth muscle cells under normal or disease conditions in vitro.
- Cryopreserved: Cryogenic vial containing 500.000 viable cells.
- Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
- Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
|Recommended plating density||7,500 – 10,000 cells per cm2|
|Passage After Thawing||P2|
|Tested Markers||Smooth muscle α-Actin positive|
|Guaranteed Population Doubling||> 15|
Technical Library (1)
From which portion of the aorta does PromoCell isolate the HAoEC and HAoSMC?
The aorta can be divided into the ascending aorta, the arch, and the descending aorta. We supply aortic cells (HAoEC, HAoSMC, HAoAF) of either "thoracic" or "abdominal" origin. In case of "thoracic" origin, we use tissue from the Aorta ascendens up to the aortic arch; in case of "abdominal" origin, we use tissue from the descending portion starting at the Diaphragma, i.e. from the Aorta abdominalis.
Please check the lot-specific Certificate of Analysis (Tissue/Localization) or contact the PromoCell Technical Customer Service if you need cells from a particular part of the aorta.
Related Links and Documents
- From which portion of the aorta does PromoCell isolate the HAoEC and HAoSMC?
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Reference Literature (50)
NCK associated protein 1 modulated by miRNA-214 determines vascular smooth muscle cell migration, proliferation, and neointima hyperplasia
Afzal et al.; J Am Heart Assoc. 2016 Dec 7;5(12)
miR-503 inhibits platelet-derived growth factor-induced human aortic vascular smooth muscle cell proliferation and migration through targeting the insulin receptor
Bi et al.;Biomed Pharmacother. 2016 Dec;84:1711-1716
Dicer generates a regulatory microRNA network in smooth muscle cells that limits neointima formation during vascular repair
Zahedi et al.; Cell Mol Life Sci. 2017 Jan;74(2):359-372
Proteomic analysis of vascular smooth muscle cells in physiological condition and in pulmonary arterial hypertension: toward contractile versus synthetic phenotypes
Regent et al.; Proteomics. 2016 Oct;16(20):2637-2649
Gax regulates human vascular smooth muscle cell phenotypic modulation and vascular remodeling
Zheng et al.; Am J Transl Res. 2016 Jul 15;8(7):2912-25
RANKL promotes osteoblastic activity in vascular smooth muscle cells by upregulating endothelial BMP-2 release
Davenport et al.; Int J Biochem Cell Biol. 2016 Jun 23;77(Pt A):171-180
Activated mineralocorticoid receptor regulates micro-RNA-29b in vascular smooth muscle cells
Bretschneider et al.; FASEB J. 2016 Apr;30(4):1610-22
High serum CXCL10 in Rickettsia conorii infection is endothelial cell mediated subsequent to whole blood activation
Otterdal et al.; Cytokine. 2016 Jul;83:269-74
High-throughput RNAi screening identifies a role for the osteopontin pathway in proliferation and migration of human aortic smooth muscle cells
Zhang et al.; Cardiovasc Drugs Ther. 2016 Jun;30(3):281-95
Comparisons with amyloid-beta reveal an aspartate residue that stabilizes fibrils of the aortic amyloid peptide medin
Davies et al.; J Biol Chem. 2015 Mar 20;290(12):7791-803
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