Human Aortic Endothelial Cells (HAoEC)
Primary Human Aortic Endothelial Cells isolated from the human ascending and descending aorta.
Primary Human Aortic Endothelial Cells (HAoEC) are isolated from the human ascending (thoracic) and descending (abdominal) aorta. They are useful for studying vascular diseases such as thrombosis, atherosclerosis, and hypertension as well as for stent-graft compatibility testing.
- Cryopreserved: Cryogenic vial containing 500.000 viable cells.
- Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
- Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
|Recommended plating density||5,000 – 10,000 cells per cm2|
|Passage After Thawing||P2|
|Tested Markers||von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positive|
|Guaranteed Population Doubling||> 15|
Technical Library (3)
From which portion of the aorta does PromoCell isolate the HAoEC and HAoSMC?
The aorta can be divided into the ascending aorta, the arch, and the descending aorta. We supply aortic cells (HAoEC, HAoSMC, HAoAF) of either "thoracic" or "abdominal" origin. In case of "thoracic" origin, we use tissue from the Aorta ascendens up to the aortic arch; in case of "abdominal" origin, we use tissue from the descending portion starting at the Diaphragma, i.e. from the Aorta abdominalis.
Please check the lot-specific Certificate of Analysis (Tissue/Localization) or contact the PromoCell Technical Customer Service if you need cells from a particular part of the aorta.
Related Links and Documents
Which medium does PromoCell recommend for endothelial cell transfection ?
There are several factors that can influence successful transfections, e.g. viability and density of the cells, choice of the transfection reagent, quality and type of the transfected molecules (plasmids, siRNA, oligonucleotides), as well as the culture medium and supplements used.
When using PromoCell Endothelial Cell Growth Media for cell transfection, please follow the instructions below:
Heparin, which is included in our Endothelial Cell Growth Medium (C-22010/C-22110), Medium 2 (C-22011/C-22111) and Medium MV (C-22020/C-22120) may reduce the transfection efficiency. We therefore recommend to use our heparin-free Endothelial Cell Growth Medium MV2 (C-22022/C-22121). Alternatively, you may use Endothelial Cell Growth Medium Kit (C-22110), Medium 2 Kit (C-22111), or Medium MV Kit (C-22120) without adding the heparin supplement to the Basal Medium.
Please note: Before and after transfection, the cells should be cultured in complete Growth Medium including heparin to ensure optimal growth.
Related Links and Documents
What type of Endothelial Cells should I use to study atherosclerosis?
Atherosclerosis refers to the buildup of fats in and on artery walls (plaques), which can restrict blood flow. These plaques can also burst, causing a blood clot. Although atherosclerosis is often considered a heart problem, it can affect arteries anywhere in the body. PromoCell supply HCAEC (endothelial cells from coronary artery), HAoEC (EC from human aorta), HPAEC (EC from pulmonary artery) that can be used to study atherosclerosis.
Related Links and Documents
- From which portion of the aorta does PromoCell isolate the HAoEC and HAoSMC?
Application Notes (0)
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Reference Literature (64)
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Colon cancer-derived myofibroblasts increase endothelial cell migration by glucocorticoid-sensitive secretion of a pro-migratory factor
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The endocannabinoid anandamide causes endothelium-dependent vasorelaxation in human mesenteric arteries
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RANKL promotes osteoblastic activity in vascular smooth muscle cells by upregulating endothelial BMP-2 release
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A disrupted endothelial cell layer and exposed extracellular matrix proteins promotes capture of late outgrowth endothelial progenitor cells
Zhao et al.; Stem Cells International. 2016;2016:Article ID 1406304, 13 pages
PGC-1alpha ameliorates AngiotensinII-induced eNOS dysfunction in human aortic endothelial cells
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Human Primary Cells in Drug DevelopmentFacilitate the path to an approved new medicine: It takes roughly 14 years to take a substance from preclinical stage to the bedside. To generate all of the knowledge needed to register a new compound, you need many experiments and different assays. Find out how primary cells might help you with this. (DINA1: 594 x 841 mm; PDF)
Endothelial Cell TypesWant to know more about our portfolio of endothelial cells? Take a look at our poster for an overview of our large-vessel and microvascular endothelial cells, and where they are located in the body.
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