Human Aortic Adventitial Fibroblasts (HAoAF)
Primary Human Aortic Adventitial Fibroblasts isolated from the human aortic adventitial tissue.
Primary Human Aortic Adventitial Fibroblasts (HAoAF) are isolated from human aortic adventitial tissue. HAoAF can be used for studies concerning vascular diseases e.g. neointimal formation, vascular remodeling, atherosclerosis, and hypertension. They are also often used for the examination of fibroblast disorders like fibrosis or other diseases linked to either imperfect or excessive accumulation of fibroblasts.
- Cryopreserved: Cryogenic vial containing 500.000 viable cells.
- Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
- Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
|Recommended Plating Density||3.500 – 7.000 cells per cm2|
|Passage After Thawing||P2|
|Tested Markers||CD90 positive|
|Guaranteed Population Doubling||> 10|
|Guaranteed Population Doublings||> 10|
Technical Library (2)
What is the exact localization of PromoCell’s HAoAF (Human Aortic Adventitial Fibroblasts)?
Our HAoAF (C-12380) are isolated from the Adventitia, the outer layer of the aorta. The cells have been characterized as fibroblasts by the expression of fibroblast-specific CD90.
Related Links and Documents
From which portion of the aorta does PromoCell isolate the HAoEC and HAoSMC?
The aorta can be divided into the ascending aorta, the arch, and the descending aorta. We supply aortic cells (HAoEC, HAoSMC, HAoAF) of either "thoracic" or "abdominal" origin. In case of "thoracic" origin, we use tissue from the Aorta ascendens up to the aortic arch; in case of "abdominal" origin, we use tissue from the descending portion starting at the Diaphragma, i.e. from the Aorta abdominalis.
Please check the lot-specific Certificate of Analysis (Tissue/Localization) or contact the PromoCell Technical Customer Service if you need cells from a particular part of the aorta.
Related Links and Documents
- What is the exact localization of PromoCell’s HAoAF (Human Aortic Adventitial Fibroblasts)?
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Reference Literature (3)
Construction and histological analysis of a 3D human arterial wall model containing vasa vasorum using a layer-by-layer technique
Shima et al.; J Biomed Mater Res A. 2017 Mar;105(3):814-823
Collagen matrix stiffness influences fibroblast contraction force
Jin et al.; Biomed. Phys. Eng. Express 2 (2016) 047002
A novel collagen gel-based measurement technique for quantitation of cell contraction force
Jin et al.; J R Soc Interface. 2015 May 6;12(106)
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